돼지 액상 정액의 보관일수에 따른 오염 정도가 체외 수정란 생산 효율에 미치는 영향

서지정보

· 발행기관 : 한국수정란이식학회
· 수록지 정보 : Journal of Embryo Transfer / 21권 / 4호
· 저자명 : 김연수, 이현택, 김인철, 유재원, 김철욱, 정기화

초록

본 연구는 항생제가 첨가되지 않은 돼지 혼합액상 정액을 17 정액 보관고에 보관하면서 보관일수의 증가가 따라 정자의 운동성, 정액 내 세균의 증식 여부 및 체외 수정란 생산 효율에 미치는 영향을 조사하고자 하였다. 정자의 운동성은 1일 (78.72.4%)에 비하여 3일(78.72.4%)과 5일째(64.82.4%)는 유의적으로(p<0.05) 낮은 운동성을 나타내었다. 보관일수에 따른 정액 내 세균수의 변화는 보관 5일이 Cfu로 0일과 3일의 Cfu와 C

영어초록

The objective of this study was to investigate the effects of preservation period of porcine liquid semen on bacterial contamination and in vitro production of embryo. Extended liquid semen was prepared by three mixture of boar's ejaculates from each farm without antibiotics, and were kept in semen preservation incubator until use. Sperm motility was significantly (p<0.05) decreased as semen preservation time goes by (78.72.4% for 1 day vs. 71.12.4 and 64.82.4% for 3 and 5 days of presentation, respectively). Quantitative of bacteria in semen was significantly (p<0.05) higher in 5 days ( Cfu) compared to 0 and 3 days ( and Cfu, respectively) of preservation. In terms of development of in vitro fertilization of porcine embryos inseminated by preserved semen, the rate of normal fertilization (2PN) was significantly (p<0.05) decreased in 5 days (56.02.6%) compared to 1 and 3 days (66.02.7 and 64.02.7%, respectively) of preservation. Cleavage rate was also significantly (p<0.05) affected by preservation period (75.04% for 1 day, 70.00.3 and 71.00.3% for 3 and 5 days, respectively). The in vitro developmental rate of blastocyst stage embryo was significantly (p<0.05) affected by semen preservation period (15.01.0% for 1 day vs. 11.00.9 and 8.00.9% for 3 and 5 days, respectively). It is concluded that more than 3 days of liquid semen preservation without antibiotics increased the quantity of bacteria resulted in detrimental effect on sperm motility and decreased both normal insemination rate and the developmental rate of blastocyst stage embryo.

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