한우 체외 수정란의 배반포 단계, 체외 배양 일령 및 등급이 산자의 생산에 미치는 영향

서지정보

ㆍ발행기관 : 한국수정란이식학회 ㆍ수록지정보 : Journal of Embryo Transfer / 21권 / 4호
ㆍ저자명 : 박용수, 김소섭, 김동환, 박흠대

한국어 초록

본 연구는 수정란이식의 현장 적용 측면을 중심으로, 한우 체외 수정란의 발생 단계, 일자 및 등급이 젖소 대리모에 이식 후 임신과 유산, 분만과 관련된 임신기간, 생시체중, 난산 및 쌍자율을 조사하였다. 또한 단자 및 쌍자 분만에 따른 임신기간, 생시체중, 성비 및 폐사율을 분석하였다. 발생 단계에 따른 임신율은 배반포 단계(64.4%), 유산율은 부화 배반포(HB) 단계(21.4%)가 유의하게 높았다(p<0.05). 발생 일령에 따른 임신율은 7일령

영어 초록

This study examined the effects of the in vitro produced (IVP) Hanwoo blastocyst stage (blastocyst, expanded blastocyst and hatched blastocyst), in vitro culture day (7, 8, and 9) and blastocyst grade (1, 2 and 3) on the pregnancy rate, gestation length, birth weight, the incidence of dystocia and twining rate after embryo transfer (ET). The pregnancy and abortion rates were significantly higher in the blastocyst (B) stage (64.4%) and in the hatched blastocyst (HB) stage (21.4%), respectively, than in those of the other developmental stages (p<0.05). The pregnancy rate of Day 7 embryos (49.0%) was significantly higher than those of Days 8 and 9 embryos (36.4 and 15.4%), but the abortion rates were similar (0 to 10.7%). There were no significant differences in the pregnancy (41.4 to 42.5%) and abortion (9.3 to 16.5%) rates among the three grades of embryos. There were no significant differences in gestation length, birth weight and the incidence of dystocia among the three development stages, but the twinning rate was significantly higher in the HB stage (p<0.05). The pregnancy rate, the incidence rate of dystocia and twinning rate were similar among the three different culture days, however birth weight was significantly heavier in calves from Day 9 embryos than in those from Days 7 and 8 embryos. The mean gestation length of grades 1 and 2 embryos (278.5 and 276.1 days) were significantly longer than that of grade 3 (p<0.05), but birth weight, the incidence of dystocia and twinning rate did not significantly differ. The mean gestation length in single calves was significantly longer than that in twin calves (278.5 vs. 272.5 days, p<0.05). In addition, the mean birth weight in single calves was significantly greater than that in twin calves (29.6 vs. 22.3 kg, p<0.05). Finally, the sex ratios and mean mortality rates between single and twin calves were similar.

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