제주흑우 동결정액 제조에 있어 Low Density Lipoproteins (LDL)과 항산화제로서 Taurine, Hypotaurine 그리고 Trehalose 조합이 동결 융해 후 정자의 성상에 미치는 영향

서지정보

· 발행기관 : 한국동물번식학회
· 수록지 정보 : Reproductive & developmental biology / 36권 / 3호
· 저자명 : 오신애, 고민회, 강태영, 최선호, 고문석, 오영미, 조원모

목차

ABSTRACT 서론 제주흑우 정액 채취 정액의 처리 및 제조 정자의 운동성 평가 정자의 생존율 평가 정자막 온전성 평가 첨체막 변화 양상의 측정 정자의 난자내 침투 능력 평가 통계 분석 결과 LDL과 항산화제가 동결 융해 후 정자의 운동성과 생존율에 미치는 영향 LDL과 항산화제의 조합이 동결 융해 후 정자막 온전성에 미치는 영향 LDI과 항산화제의 조합이 동결 융해 후 정자의 첨체막 변화에 미치는 영향 LDL과 항산화제의 조합이 동결 융해 후 정자의 수정능력에 미치는 영향 고찰 적요 인용문헌

영어초록

This study was designed to determine whether low-density lipoproteins (LDL) from egg yolk and taurine, hypotaurine and trehalose as antioxidant in extender improve the freezability and fertility of Korean Jeju Black Bull semen. The semen was cryopreserved with tris egg yolk extenders containing 7% glycerol and treated 4% LDL, 20 mM taurine, hypotaurine and trehalose. Frozen-thawed sperm were evaluated motility, viability, membrane, and acrosome integrity and sperm penetration ability. The results were compared to semen cryopreserved in tris egg yolk extender only as control. Frozen-thawed semen evaluation cleary indicated that the addition of LDL and LDL-antioxidants (taurine, hypotaurine and trehalose) combination were significantly improved (p<0.05) the viability (%; with staining test using eosin-Y) compared to control spermatozoa. Also, in membrane integrity (%; with supravital hypo-osmotic swelling test), not only LDL-antioxiants combination but also LDL were significantly increased (p<0.05) the swelled sperm using HOST compared to control. Sperm acrosome integrity state was classified by CTC (chlortetracycline) staining test. F pattern was significantly increased in LDL-antioxidant combination than control (p<0.05) and B pattern was not significantly differences among all treatments and control. However, AR pattern was significantly decreased in LDL-antioxidants combination than control (p<0.05). Pronucleus formation and sperm penetration index (SFI) were significantly increased in LDL and LDL-antioxidants combination than control (p<0.05). Especially, LDL-taurine significantly improved pronucleus fomation and SFI than LDL (p<0.05). It was concluded that LDL and LDL-antioxidants in extender improved the freezability and fertility of Korean Jeju Black bull spermatozoa.

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