The boar semen was collected by gloved-hand method and cryopreserved in 20% egg yolk freezing extender ... , and cryopreservation efficiency of boar semen would be improved by use of ALA. ... In conclusion, this results show that supplement of ALA during the cryopreservation process could reduce
To preserve genetic materials, cryopreservation of the semen from live animals is the main technique ... cauda epididymis (CE) and electro-stimulated semen (ES). ... When seminal plasma of fresh ES semen was washed with semen washing media (SWM), the rates of live sperm
by lowing the straws into styrofoam box on the 8cm above the LN2 and plunged into LN2 for cryopreservation ... These results of this study indicate that supplementation of 0.5% OEP in boar semen cryo-extender can ... during freezing in boar semen.
The aim of this study was to evaluate the effects of different straw volume (0.5 and 5.0 ㎖) on cryopreservation ... other semen characteristic and developmental rate of IVM/IVF embryos. ... in boar semen.
The boar semen was collected by gloved-hand method and cryopreserved using freezing extender containing ... In conclusion, our findings suggest that quality of frozen-thawed sperm in mammalians could improve by ... Cryopreserved boar sperms were thawed in 37C water-bath for 45 sec to analysis.
The ejaculated semen was diluted with Triladyl containing 20% egg-yolk and cryopreserved in liquid notrigen ... In couclusion, we suggest that using the thawing medium containing NA for sperm dilution can be benefical ... The characteristics of frozen-thawed sperm were estimated with SYBR14/PI double staining for viability
The results were compared to semen cryopreserved in tris egg yolk extender only as control. ... The semen was cryopreserved with tris egg yolk extenders containing 7% glycerol and treated 4% LDL, 20 ... Frozen-thawed semen evaluation cleary indicated that the addition of LDL and LDL-antioxidants (taurine
The aim of present experiment was to examine commercial synthetic extender(AndroMed) for semen cryopreservation ... The present results that acceptable fertilization and cryopreservation could be obtained by in vitro ... The semen straws were located above 5 cm of liquid nitrogen for 5 minutes, above 5 cm for 10 minutes
The aim of this study was to evaluate the post-thawed characteristics of leopard cat semen. ... The present results concluded that leopard cat semen can be collected successfully by electro-ejaculation ... method and cryopreserved successfullyfor future use in different assisted reproductive technologies.
In conclusion, GalT KO cloned miniature pig semen can be cryopreserved successfully and used for artificial ... The purpose of this study was undertaken to evaluate of cryopreservation efficiency in α 1,3-galactosyltransferase ... The ejaculated semen from the three pig species was diluted with same volume extender and added to LEY
semen cryopreservation for enhance reproductive technology and animal genetic resources conservation ... freezing, slow-freezing and vitrification methods were used as a cryopreservation. ... After 44 h of culture, the oocytes were inseminated with frozen-thawed semen and fresh semen prepared
Miniature pig sperm cryopreservation is continually researched in biotechnology for breed conservation ... It is important to control the temperature at each stage of cryopreservation and cryoprotectant. ... Miniature pig sperm was separated from semen by washing with modified- Modena B (mMB) extender.
, 209 mM; citric acid, 6 mM; glucose, 5 mM; glycerol, 8%) and cryopreserved gradually by slow-cooling ... with ejaculated fresh semen and frozen-thawed semen. ... However, frozen-thawed semen can be used successfully fur artificial insemination in dog.
The objective of this study was to examine effect of ethylene glycol for semen cryopreservation in Korean ... The semen was cryopreserved with extenders containing cryoprotectants (7% glycerol and 3%, 5%, 7% ethylene ... The semen straws were located above 3 cm of liquid nitrogen for 5 min, 5 cm for 10 min and 8 cm for 10
Triladyl and LEY containing egg yolk to the cryopreservation of separated sperm by percoll in miniature ... The ejaculated semen from miniature pig was separated by 65% percoll and un-separated sperm as a control ... The groups were designed that as separated sperm by Percoll with Triladyl(ST) or LEY(SL) for cryopreservation
We report herein the successful results of estrus induction, sperm cryopreservation and kids born by ... The concentration of frozen-thawed semen was and 0.5 ml of frozen-thawed semen was transcervically inseminated ... transcervical insemination of frozen-thawed semen in a Saanen goat.
the LN2 vapor over 5 cm above from LN2 and then immersed directly in LN2 for cryopreservation. ... of post-thaw motility and so both semen collection methods could be applied in feline semen collection ... the post-thaw viability of cat semen.
This study was carried out to investigate synthetic extender for semen cryopreservation of Jeju Native ... The semen straws were located above 5 cm for 10 minutes. ... The semen was equilibrated for 4 hours at cold chamber and packed to 0.5 ml straw.
and/or L-cysteine (L), catalase (C) and L-cysteine + catalase was added to the diluted semen for cryopreservation ... damage during sperm cryopreservation. ... The semen from bulls was collected by the artificial vagina method, and Triladyl containing 20% egg-yolk
The semen straws were located above 5 cm of liquid nitrogen for 5 minutes. ... The semen was equilibrated for 1 hrs at cold chamber and packed to 0.5 ml straw. ... was conducted to investigate the survival rate of AndroMed and Tris-egg yolk extender for cryopreservation