[생물공정실험] 1주차 Mammalian suspension cell culture 예비보고서

본 내용은 " [생물공정실험] 1주차 Mammalian suspension cell culture 예비보고서 " 의 원문 자료에서 일부 인용된 것입니다. 2023.01.16

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• 1. Mammalian Suspension cell culture

  이 보고서는 Mammalian cell을 suspension culture로 배양하고 세포 수를 측정하여 viability를 확인하는 실험에 대한 내용을 다루고 있습니다. 실험 이론에서는 subculture, mammalian cell culture, adherent cell vs suspension cell, viability 측정 방법 등을 설명하고 있으며, CHO cell과 HEK cell 등 다양한 mammalian cell 라인에 대해서도 소개하고 있습니다. 또한 유전자 가위 기술의 발전 과정도 간략히 다루고 있습니다.

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• 1. Mammalian Suspension cell culture

  Mammalian suspension cell culture is a widely used technique in the field of biotechnology and pharmaceutical research. This method allows for the large-scale production of various proteins, antibodies, and other biomolecules derived from mammalian cells. The key advantage of suspension cell culture is the ability to grow cells in a controlled, scalable environment without the need for adherent surfaces, enabling higher cell densities and improved productivity compared to traditional adherent cell culture methods. One of the primary benefits of mammalian suspension cell culture is its versatility. It can be used to produce a wide range of therapeutic proteins, including monoclonal antibodies, recombinant enzymes, and vaccines, which are critical for the development of new drugs and treatments. The ability to grow cells in suspension also simplifies the downstream processing and purification of the desired biomolecules, making the overall production process more efficient and cost-effective. Furthermore, suspension cell culture systems can be easily scaled up to meet the increasing demand for biopharmaceuticals, as they can be readily adapted to larger bioreactors and fermentation systems. This scalability is particularly important in the context of rapidly evolving healthcare needs and the growing global market for biologics. However, the successful implementation of mammalian suspension cell culture requires careful optimization of various parameters, such as media composition, pH, temperature, and dissolved oxygen levels, to ensure optimal cell growth, viability, and product yield. Additionally, the maintenance of sterile conditions and the prevention of contamination are crucial aspects of this technology, as mammalian cells are more sensitive to environmental factors compared to microbial systems. Overall, mammalian suspension cell culture is a powerful and versatile tool that has significantly contributed to the advancement of biopharmaceutical production and the development of innovative therapies. As the demand for biologics continues to grow, this technology will likely play an increasingly important role in the future of the healthcare industry.