3D cell culture system
- 최초 등록일
- 2021.06.15
- 최종 저작일
- 2017.04
- 17페이지/ MS 파워포인트
- 가격 2,000원
소개글
"3D cell culture system"에 대한 내용입니다.
목차
1. Why 3D cell cultures?
2. 2D vs 3D cell culture
3. Method of spheroids
4. Bioinspired tuning of glycol chitosan for 3D cell culture
본문내용
Hanging-drop method
• Cell suspension drops are deposited onto the underside of the lid of a tissue culture dish. Each drop is 15–30 μL in volume containing approximately 300–3000 cells. When the lid is inverted, drops are held in place by surface tension.
• Advantages : Well-controlled spheroid size, Fast spheroid formation
• Disadvantages : Labor intensive
Large-scale spheroid production in rotary bioreactors
• Spinner flasks and roller bottles with appropriate surface treatment can also be used to generate spheroid in large quantities. The average spheroid diameter can be controlled by tuning cell-seeding density, medium composition, spinning rate and culture time.
참고 자료
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