주령별 쥐 대뇌피질 신경 조직의 초기 발달단계에서 UDP-GlcNAc 함량 및 단백질의 O-GlcNAc 수식 변화 (O-GlcNAcylation of Proteins and UDP-GlcNAc Content in Rat Brain Cortex Tissue Changes during Early Post-natal Development)

서지정보

· 발행기관 : 한국키틴키토산학회
· 수록지 정보 : Journal of Chitin and Chitosan / 14권 / 4호 / 214 ~ 219페이지
· 저자명 : 이윤희, 김성민, 도수일, 추영국, 박용일, 박제권, 김성욱

초록

The extrinsic glucose supply, the biosynthesis of UDP-GlcNAc, protein O-GlcNAcylation and sialylation of cell surface proteins
are now increasingly important aspects for normal function of brain neuronal system. As a preliminary study, we investigated
changes in protein O-GlcNAcylation, sialylation and the content of UDP-GlcNAc in the rat brain cortical tissues of different ages,
embryonic(E18), new-born(P0), 4-week(P4W) and 8-week-old(P8W) rats. As the rats grow up to young adult(8-week-old), OGlcNAcylation
in total proteins and number of protein species gradually increased along with sharp decrease in UDP-GlcNAc
content. In contrast to O-GlcNAcylation, total sialic acid content in total proteins of each tissue showed an increase from E18 to P0
and then gradually decreased. These results may suggest that UDP-GlcNAc metabolism and protein O-GlcNAc modification and
sialic acid expression are controlled by rather complicate mechanism during the normal development of early post-natal brain
tissues. Elucidating the mechanisms of the metabolic processes between these factors and their regulation would be beneficial for
the prevention and treatment of neurodegenerative diseases such as hereditary and Alzheimer’s diseases.

영어초록

The extrinsic glucose supply, the biosynthesis of UDP-GlcNAc, protein O-GlcNAcylation and sialylation of cell surface proteins
are now increasingly important aspects for normal function of brain neuronal system. As a preliminary study, we investigated
changes in protein O-GlcNAcylation, sialylation and the content of UDP-GlcNAc in the rat brain cortical tissues of different ages,
embryonic(E18), new-born(P0), 4-week(P4W) and 8-week-old(P8W) rats. As the rats grow up to young adult(8-week-old), OGlcNAcylation
in total proteins and number of protein species gradually increased along with sharp decrease in UDP-GlcNAc
content. In contrast to O-GlcNAcylation, total sialic acid content in total proteins of each tissue showed an increase from E18 to P0
and then gradually decreased. These results may suggest that UDP-GlcNAc metabolism and protein O-GlcNAc modification and
sialic acid expression are controlled by rather complicate mechanism during the normal development of early post-natal brain
tissues. Elucidating the mechanisms of the metabolic processes between these factors and their regulation would be beneficial for
the prevention and treatment of neurodegenerative diseases such as hereditary and Alzheimer’s diseases.

참고자료

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