옥수수수염에 함유된 Flavonoids의 분리 및 동정 (Isolation and Identification of Flavonoids from Corn Silk)

서지정보

· 발행기관 : 한국작물학회
· 수록지 정보 : 한국작물학회지 / 59권 / 4호 / 435 ~ 444페이지
· 저자명 : 김선림, 김미정, 이유영, 정건호, 손범영, 이진석, 권영업, 박용일

초록

This study was carried out to isolate and characterizethe flavonoids present in corn silks. Maysin content in theunpollinated corn silks (Kwangpyeongok) showed its highestlevel at 3 days after silking, and decreased thereafter, whilethe content of open pollinated silks were consistently decreasedafter silking. This result indicates that the maysin contentis considerably affected by the pollination of corn silk.
Unpollinated corn silks were collected with excising, andethanol employed to extract flavonoids at common temperaturefor 9 days. After extraction, chlorophyll, lipids etc. wereremoved with methylene chloride, then submitted to flashcolumn cartridge (150 × 40 mm i.d.) packed with a preparativeRP-C18 bulk packing material (125 Å, 55-105 μm), andmonitored at 352 nm. Four fractions, fraction-I, -II, -III,and -IV, were isolated from ethanolic extract of corn silks.
Absorption spectrum of fraction I showed its maximum intensity(λmax) at 327 nm and 239 nm, fraction-II showed its maximumintensity at 339 nm and 274 nm, fraction-III showed itsmaximum intensity at 345 nm and 277 nm, and fraction-IVshowed its maximum intensity at 352 nm, 270 nm, 257 nm,respectively. On the baisis of ESI micro-TOF analysis, fraction-Iwas identified as chlorogenic acid (m/z 355, 3-(3,4-dihydroxycinnamoyl)quinic acid, C16H18O9), fraction-II identifiedas a mixture of chlorogenic acid and luteolin 3'-methylether 7-glucuronosyl-(1→2)-glucuronide (m/z 653, C28H28O18),fraction-III identified as a mixture of chlorogenic acid luteolin7-O-neohesperidoside (m/z 595, C27H30O15), and luteolin 3'-methylether 7-glucuronosyl-(1→2)-glucuronide, and fraction-IV identifiedas maysin (m/z 577, 2''-O-α-L-rhamnosyl-6-C-(6-deoxy-xylohexose-4-ulosyl)luteolin, C27H28O14), respectively. From theethanolic extract of corn silks, fraction-I was obtained about35 mg/100 g F.W., fraction-II was about 48 mg/100 g F.W.,fraction-III was about 46 mg/100 g F.W., and fraction-IVwas about 138 mg/100 g F.W., respectively

영어초록

This study was carried out to isolate and characterizethe flavonoids present in corn silks. Maysin content in theunpollinated corn silks (Kwangpyeongok) showed its highestlevel at 3 days after silking, and decreased thereafter, whilethe content of open pollinated silks were consistently decreasedafter silking. This result indicates that the maysin contentis considerably affected by the pollination of corn silk.
Unpollinated corn silks were collected with excising, andethanol employed to extract flavonoids at common temperaturefor 9 days. After extraction, chlorophyll, lipids etc. wereremoved with methylene chloride, then submitted to flashcolumn cartridge (150 × 40 mm i.d.) packed with a preparativeRP-C18 bulk packing material (125 Å, 55-105 μm), andmonitored at 352 nm. Four fractions, fraction-I, -II, -III,and -IV, were isolated from ethanolic extract of corn silks.
Absorption spectrum of fraction I showed its maximum intensity(λmax) at 327 nm and 239 nm, fraction-II showed its maximumintensity at 339 nm and 274 nm, fraction-III showed itsmaximum intensity at 345 nm and 277 nm, and fraction-IVshowed its maximum intensity at 352 nm, 270 nm, 257 nm,respectively. On the baisis of ESI micro-TOF analysis, fraction-Iwas identified as chlorogenic acid (m/z 355, 3-(3,4-dihydroxycinnamoyl)quinic acid, C16H18O9), fraction-II identifiedas a mixture of chlorogenic acid and luteolin 3'-methylether 7-glucuronosyl-(1→2)-glucuronide (m/z 653, C28H28O18),fraction-III identified as a mixture of chlorogenic acid luteolin7-O-neohesperidoside (m/z 595, C27H30O15), and luteolin 3'-methylether 7-glucuronosyl-(1→2)-glucuronide, and fraction-IV identifiedas maysin (m/z 577, 2''-O-α-L-rhamnosyl-6-C-(6-deoxy-xylohexose-4-ulosyl)luteolin, C27H28O14), respectively. From theethanolic extract of corn silks, fraction-I was obtained about35 mg/100 g F.W., fraction-II was about 48 mg/100 g F.W.,fraction-III was about 46 mg/100 g F.W., and fraction-IVwas about 138 mg/100 g F.W., respectively

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