太陰人 淸心蓮子湯이 Hydrogen Peroxide에 損傷)된 白鼠의 大腦神經細胞에 미치는 影響 (Effects of Taeumin Chungsimyeunjatang on the Cerebral neurons injured by Hydrogen Peroxide)

서지정보

· 발행기관 : 사상체질면역의학회
· 수록지 정보 : 사상체질면역의학회지 / 11권 / 2호 / 251 ~ 266페이지
· 저자명 : 옥윤영, 류도곤, 김경요

초록

1. Purpose : The purpose of this study was to determine the effects of Chungsimyeunjatang on the cerebral neurons injured by hydrogen peroxide(H₂O₂).
2. Methods : I observed cell viability in mouse cerebral neurons exposed to hydrogen peroxide by NR assay and MTT assay and determined lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide.
After administration of Chungsimyeunjatang water extracts, I observed significant changes of cell viability, lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide.
3. Results : Hydrogen peroxide showed neurotoxicity.
Cell viability in mouse cerebral neurons exposed to hydrogen peroxide decreased in NR assay and MTT assay.
Lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide increased.
Chungsimyeunjatang was very effective in blocking hydrogen peroxide-induced neurotoxicity.


1. 연구목적본 실험은 청심연자탕이 대뇌신경세포의 산화적 손상에 대한 효능을 밝히기 위한 것이다.
2. 연구방법신생 생쥐에서 분리 배양한 대뇌신경세포에 여러 농도의 hydrogen peroxide가 포함된 배양액에서 6시간 동안 처리하여 hydrogen peroxide가 배양 대뇌신경세포에 미치는 영향을 조사하였으며 hydrogen peroxide의 독성효과에 대한 청심연자당의 영향을 조사하였다.
3. 결과 및 결론산소자유기인 hydrogen peroxide는 NR assay와 MTT assay에 의한 세포생존율을 감소시켰고 lipid peroxidation의 증가 및 LDH양의 증가에 의하여 생쥐의 배양 대뇌신경세포에 독성을 나타냈다.
淸心蓮子湯은 hydrogen peroxide의 산화적 손상에 대한 신경독성에 대하여 Iipid peroxidation의 감소에 유의한 효과를 보였다. 淸心蓮子湯은 hydrogen peroxide의 산화적 손상에 의한 신경독성에 대하여 LDH양의 감소에 유의한 효과를 보였다.
이상의 결과로 보아 hydrogen peroxide는 생쥐에서 분리한 대뇌신경세포에 산화적 손상에 의한 신경독성을 나타냈으며 청심연자탕(淸心蓮子湯)이 hydrogen peroxide와 같은 산소자유기의 산화적 손상에 대한 방어에 효과적인 것으로 사료된다.

영어초록

1. Purpose : The purpose of this study was to determine the effects of Chungsimyeunjatang on the cerebral neurons injured by hydrogen peroxide(H₂O₂).
2. Methods : I observed cell viability in mouse cerebral neurons exposed to hydrogen peroxide by NR assay and MTT assay and determined lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide.
After administration of Chungsimyeunjatang water extracts, I observed significant changes of cell viability, lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide.
3. Results : Hydrogen peroxide showed neurotoxicity.
Cell viability in mouse cerebral neurons exposed to hydrogen peroxide decreased in NR assay and MTT assay.
Lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide increased.
Chungsimyeunjatang was very effective in blocking hydrogen peroxide-induced neurotoxicity.

참고자료

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