표면 적심성에 따른 NIH/3T3 섬유아세포의 생물학적 평가 (Biological evaluatin of NIH/3T3 fibroblast cells with different wettability)

서지정보

· 발행기관 : 한국조직공학과 재생의학회
· 수록지 정보 : 조직공학과 재생의학 / 3권 / 1호 / 58 ~ 63페이지
· 저자명 : 김선경, 조영호, 조미희, 신유나, 김문석, 강길선, 이봉, 이해방

초록

The modification of biomaterial surface using various surface treatment methods has recently become an
interesting topic. In this study, a low density polyethylene (LDPE) film was treated by corona discharge method.
Three kinds of different wettability on LDPE surface such as 70o, 55o and 45o of water contact angle were prepared
by the control of sample bed speed of corona treatment apparatus. The adhered cell morphology of NIH/3T3 fibroblasts
was observed by scanning electron microscope and the cell viability was evaluation by MTT assay. The evaluation
of mRNA expression of cells contacting with LDPE film was measured by a reverse transcription-polymerase
chain reaction (RT-PCR). At 55o wettability, the number of the cell adhered and proliferated on the surface was the
highest and specific gene of c-myc, c-fos and p53 showed the maximum expression. In conclusion, this investigation
demonstrated that the surface wettability plays an important role for gene regulation as well as cell adhesion and
proliferation behavior for biomedical application.

영어초록

The modification of biomaterial surface using various surface treatment methods has recently become an
interesting topic. In this study, a low density polyethylene (LDPE) film was treated by corona discharge method.
Three kinds of different wettability on LDPE surface such as 70o, 55o and 45o of water contact angle were prepared
by the control of sample bed speed of corona treatment apparatus. The adhered cell morphology of NIH/3T3 fibroblasts
was observed by scanning electron microscope and the cell viability was evaluation by MTT assay. The evaluation
of mRNA expression of cells contacting with LDPE film was measured by a reverse transcription-polymerase
chain reaction (RT-PCR). At 55o wettability, the number of the cell adhered and proliferated on the surface was the
highest and specific gene of c-myc, c-fos and p53 showed the maximum expression. In conclusion, this investigation
demonstrated that the surface wettability plays an important role for gene regulation as well as cell adhesion and
proliferation behavior for biomedical application.

참고자료

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