폴리감마글루탐산 및 구연산을 혼합한 인산칼슘 시멘트의 역학 및 생물학적 분석 (Mechanical and Biologic Assessment of Calcium Phosphate Cement Mixed with Poly-Gamma-Glutamic Acid and Citric Acid)

서지정보

· 발행기관 : 한국조직공학과 재생의학회
· 수록지 정보 : 조직공학과 재생의학 / 6권 / 4호 / 978 ~ 985페이지
· 저자명 : 정진화, 김정만, 김승수, 권순용

초록

The purpose of this study was to make and evaluate the injectable alpha tricalcium phosphate(α-TCP) cement mixed with poly-gamma-glutamic acid(PGGA) and citric acid. α-TCP and PGGA component powders and citric acid solution were prepared. After mixing each 5, 10 and 15 wt% PGGA with α-TCP powder, the best match was decided by mechanical study. And then studies of initial setting time, pH, released calcium ion, mechanical strength, dynamic viscosity were done after mixing each 5, 10 and 15 wt% citric acid solution with α-TCP cement incorporated with 10 wt% PGGA. Also, cell proliferation assay using osteoblast and confocal microscope were performed.
The results showed 10 wt% PGGA mixed with α-TCP cement was the best mechanical strength(compression strength; 50 MPa & diametral tensile strength; 18 MPa), but the initial setting time was about 90 minutes. After mixing various weight percents of citric acid, mixing with 10 wt% citric acid showed 80 MPa of compression strength, 22 MPa of diametral stensile strength and 5 minutes of initial setting time. As compared to control(MIIG™ 115), this match showed higher absorbance in cell proliferation assay, more individualization of actin and more activation of integrin in confocal microscope. Conclusively, α-TCP cement mixed with 10 wt% PGGA and 10 wt% citric acid showed good mechanical and biologic results. This study can be applied clinically to making injectable calcium phosphate cement bone substitute.

영어초록

The purpose of this study was to make and evaluate the injectable alpha tricalcium phosphate(α-TCP) cement mixed with poly-gamma-glutamic acid(PGGA) and citric acid. α-TCP and PGGA component powders and citric acid solution were prepared. After mixing each 5, 10 and 15 wt% PGGA with α-TCP powder, the best match was decided by mechanical study. And then studies of initial setting time, pH, released calcium ion, mechanical strength, dynamic viscosity were done after mixing each 5, 10 and 15 wt% citric acid solution with α-TCP cement incorporated with 10 wt% PGGA. Also, cell proliferation assay using osteoblast and confocal microscope were performed.
The results showed 10 wt% PGGA mixed with α-TCP cement was the best mechanical strength(compression strength; 50 MPa & diametral tensile strength; 18 MPa), but the initial setting time was about 90 minutes. After mixing various weight percents of citric acid, mixing with 10 wt% citric acid showed 80 MPa of compression strength, 22 MPa of diametral stensile strength and 5 minutes of initial setting time. As compared to control(MIIG™ 115), this match showed higher absorbance in cell proliferation assay, more individualization of actin and more activation of integrin in confocal microscope. Conclusively, α-TCP cement mixed with 10 wt% PGGA and 10 wt% citric acid showed good mechanical and biologic results. This study can be applied clinically to making injectable calcium phosphate cement bone substitute.

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