LC-MS/MS를 이용한 벌꿀 중 grayanotoxin 분석법 연구 및 실태조사 (Determination and Monitoring of Grayanotoxins in Honey Using LC-MS/MS)

서지정보

· 발행기관 : 한국식품과학회
· 수록지 정보 : 한국식품과학회지 / 40권 / 1호 / 8 ~ 14페이지
· 저자명 : 이숙연, 최윤주, 이강봉, 조태용, 김진숙, 손영욱, 박재석, 임성임, 최희정, 이동하

초록

This study was performed to establish analysis methods, and evaluated for grayanotoxin in domestic/foreignhoney and wild honey. The molecular weight of grayanotoxins I, II and II, excluding grayanotoxin II that has bencommercialized, were analyzed by LC-MS/MS. Then, the molecular structure of grayanotoxins I and II were analyzed byNMR. A total 11 samples (25 Korean honey, 21 Korean wild honey, 13 Korean honeycomb honey, 44 foreign honey, 8foreign wild honey) were examined to determined whether or not each sample contained grayanotoxins I, II, and III. Thehoney samples were mixed with methanol and loaded into a tC18 cartridge, the filtrate was diluted with water, and the mixturewas then analyzed by ESI triple-quadrupole LC-MS/MS. Grayanotoxins were only found in the foreign wild honey and werenot detected in Korean honey, Korean honeycomb honey, or Korean wild honey. Three of the samples contained grayanotoxinI, II, and III, and one sample contained only grayanotoxins I and III. The lowest level for grayanotoxin I was 3.13± 0.00 mg/kg, and the highest level was 12.93± 0.01 mg/kg. The levels of grayanotoxin II were 0.84± 0.01 mg/kg, 0.92± 0.00 mg/kgand 1.08± 0.01 mg/kg, respectively. The lowest level of grayanotoxin III was 0.25 ± 0.01 mg/kg and the highest level was3.29± 0.74 mg/kg. Through this study, safety management for foreign wild honey has been enabled.

영어초록

This study was performed to establish analysis methods, and evaluated for grayanotoxin in domestic/foreignhoney and wild honey. The molecular weight of grayanotoxins I, II and II, excluding grayanotoxin II that has bencommercialized, were analyzed by LC-MS/MS. Then, the molecular structure of grayanotoxins I and II were analyzed byNMR. A total 11 samples (25 Korean honey, 21 Korean wild honey, 13 Korean honeycomb honey, 44 foreign honey, 8foreign wild honey) were examined to determined whether or not each sample contained grayanotoxins I, II, and III. Thehoney samples were mixed with methanol and loaded into a tC18 cartridge, the filtrate was diluted with water, and the mixturewas then analyzed by ESI triple-quadrupole LC-MS/MS. Grayanotoxins were only found in the foreign wild honey and werenot detected in Korean honey, Korean honeycomb honey, or Korean wild honey. Three of the samples contained grayanotoxinI, II, and III, and one sample contained only grayanotoxins I and III. The lowest level for grayanotoxin I was 3.13± 0.00 mg/kg, and the highest level was 12.93± 0.01 mg/kg. The levels of grayanotoxin II were 0.84± 0.01 mg/kg, 0.92± 0.00 mg/kgand 1.08± 0.01 mg/kg, respectively. The lowest level of grayanotoxin III was 0.25 ± 0.01 mg/kg and the highest level was3.29± 0.74 mg/kg. Through this study, safety management for foreign wild honey has been enabled.

참고자료

· 없음