겨울철 노지 월동 중인 꿀벌과 온실 화분매개 활동 중인 꿀벌의 지방체 및 비텔로제닌 발현량 비교 분석 (Comparative Analysis of Fat Body and Vitellogenin Expression in Honey Bees Overwintering in the Field and Pollinating in a Greenhouse)

서지정보

· 발행기관 : 한국양봉학회
· 수록지 정보 : Journal of Apiculture / 40권 / 1호 / 13 ~ 20페이지
· 저자명 : 강용락, 모즈타바 에스마일리, 정철의

초록

Honey bees can be classified as summer bees with a short lifespan and winter bees with a lifespan of over 5 months based on their physiological and ecological characteristics. This compared the physiological characteristics of fat body content and vitellogenin expression levels from the honey bees overwintering in the field and pollinating inside the greenhouse during December 2024. Honey bee workers were randomly collected from 3 colonies for each environment, and their fat body ratio in the abdomen was quantified by ethyl ether extraction method, while vitellogenin expression levels were analyzed using qPCR. The fat body ratio in the abdomen showed significant difference between overwintering and pollinating honey bees. However, although there were statistically significant differences in fat body ratios among colonies, no clear trend was observed. Vitellogenin expression levels, both grouplevel and colony-level comparisons showed that overwintering colonies had significantly higher expression than pollinating colonies. Based on these findings, vitellogenin expression levels appear to be a more suitable indicator of honey bee overwintering status than the fat body contents. This study provides insight into the physiological transition of honeybees from overwintering to foraging during winter.

영어초록

Honey bees can be classified as summer bees with a short lifespan and winter bees with a lifespan of over 5 months based on their physiological and ecological characteristics. This compared the physiological characteristics of fat body content and vitellogenin expression levels from the honey bees overwintering in the field and pollinating inside the greenhouse during December 2024. Honey bee workers were randomly collected from 3 colonies for each environment, and their fat body ratio in the abdomen was quantified by ethyl ether extraction method, while vitellogenin expression levels were analyzed using qPCR. The fat body ratio in the abdomen showed significant difference between overwintering and pollinating honey bees. However, although there were statistically significant differences in fat body ratios among colonies, no clear trend was observed. Vitellogenin expression levels, both grouplevel and colony-level comparisons showed that overwintering colonies had significantly higher expression than pollinating colonies. Based on these findings, vitellogenin expression levels appear to be a more suitable indicator of honey bee overwintering status than the fat body contents. This study provides insight into the physiological transition of honeybees from overwintering to foraging during winter.

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