Scanning Electrochemical Microscopy를 이용한 한우 체내 수정란의 호흡률 조사 (Respiration Rates of Individual Bovine In Vivo-Produced Embryos Measured with a Novel, Scanning Electrochemical Microscopy)

서지정보

· 발행기관 : 사단법인 한국동물생명공학회
· 수록지 정보 : 한국동물생명공학회지 / 29권 / 1호 / 91 ~ 99페이지
· 저자명 : 김현, 복난희, 김성우, 도윤정, 김민규, 조상래, 성환후, 김동훈, 고응규

초록

Oxygen consumption is a useful parameter for evaluating mammalian embryo quality, since individual bovineembryos was noninvasively quantified by scanning electrochemical microscopy (SECM). Recently, several approacheshave been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygenconsumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, wemeasured to investigate the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo usinga SECM. In addition to, the expression of pluripotent gene and anti-oxidant enzyme was determined using real-timePCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygenconsumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos,indicating that oxygen consumption reflects the embryo quality (Grade I). Oxygen consumption of blastocyst wasmeasured using a SECM and total cell number of in vitro blastocyst was enumerated by counting cells stained bypropidium iodide. The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts(10.2 × 1015/mols—1 versus 6.4 × 1015/mols—1, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7 and 110.2in the oxygen consumption of below 10.0, 10.0∼12.0 and over 12.0∼1015/mols—1, respectively. Pregnant rate inrecipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0∼12.0 and over 12.0 × 1015/mols—1, respectively. GPX1 and SOD1 were significantly increased in over —10.0 groupthan below 10.0 groups but in catalase gene, there was no significant difference. On the other hand, In OCT-4 andSox2, pluripotent gene, there was a significant difference (p<0.05) between the below-10.0 (0.98 ± 0.1) and over 10.0(1.79 ± 0.2). In conclusion, these results suggest that measurement of oxygen consumption maybe help increase thepregnant rate of Hanwoo embryos

영어초록

Oxygen consumption is a useful parameter for evaluating mammalian embryo quality, since individual bovineembryos was noninvasively quantified by scanning electrochemical microscopy (SECM). Recently, several approacheshave been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygenconsumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, wemeasured to investigate the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo usinga SECM. In addition to, the expression of pluripotent gene and anti-oxidant enzyme was determined using real-timePCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygenconsumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos,indicating that oxygen consumption reflects the embryo quality (Grade I). Oxygen consumption of blastocyst wasmeasured using a SECM and total cell number of in vitro blastocyst was enumerated by counting cells stained bypropidium iodide. The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts(10.2 × 1015/mols—1 versus 6.4 × 1015/mols—1, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7 and 110.2in the oxygen consumption of below 10.0, 10.0∼12.0 and over 12.0∼1015/mols—1, respectively. Pregnant rate inrecipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0∼12.0 and over 12.0 × 1015/mols—1, respectively. GPX1 and SOD1 were significantly increased in over —10.0 groupthan below 10.0 groups but in catalase gene, there was no significant difference. On the other hand, In OCT-4 andSox2, pluripotent gene, there was a significant difference (p<0.05) between the below-10.0 (0.98 ± 0.1) and over 10.0(1.79 ± 0.2). In conclusion, these results suggest that measurement of oxygen consumption maybe help increase thepregnant rate of Hanwoo embryos

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