한국산 돌나물 수집종의 RAPD에 의한 유연관계 분석 (Genetic Relationship Based on RAPD Analysis of Sedum sarmentosum in Korea)

서지정보

· 발행기관 : 한국원예학회
· 수록지 정보 : 원예과학기술지 / 26권 / 1호 / 68 ~ 74페이지
· 저자명 : 김효진, 안정호, 백소현, 이승엽

초록

국내에서 수집된 31개 돌나물(Sedum sarmentosum) 지방
수집종에 대하여 15종의 10-mers와 12종의 20-mers primer
를 가지고 RAPD를 이용한 유연관계를 분석하였다. 선발된
14종의 10-mers primer로부터 47개의 polymorphic band를
Table 3. Morphological characteristics of S. sarmentosum in Korea.
Local strain Plant height (cm) Stem diameter (mm) Leaf length/width Flowering time
Wanju 19.6 ± 1.6 2.6 ± 0.3 3.5 ± 0.3 no-flowering
Gunsan 21.8 ± 1.4 2.5 ± 0.2 3.6 ± 0.5 no-flowering
Gwangyang 26.4 ± 4.2 3.1 ± 0.4 3.3 ± 0.4 May 29
Jeju 24.4 ± 2.6 3.1 ± 0.5 3.5 ± 0.4 May 30
Mokpo 28.2 ± 2.6 2.9 ± 0.4 3.3 ± 0.6 May 27
Suncheon 30.7 ± 2.4 2.7 ± 0.3 3.7 ± 0.5 May 29
Buan 30.7 ± 3.2 2.9 ± 0.3 3.3 ± 0.4 May 29
Wando 30.7 ± 2.2 3.0 ± 0.4 3.3 ± 0.2 May 28
Pohang 28.7 ± 2.6 3.0 ± 0.4 3.4 ± 0.4 May 30
Gangnung 28.2 ± 2.9 2.8 ± 0.4 3.4 ± 0.4 May 30
Kimcheon 26.3 ± 2.0 2.8 ± 0.4 3.7 ± 0.4 May 26
Gurye 26.8 ± 2.1 2.6 ± 0.3 3.3 ± 0.4 May 26
Gumi 27.2 ± 2.7 2.9 ± 0.5 3.4 ± 0.6 May 30
Nonsan 29.0 ± 2.4 2.8 ± 0.4 3.4 ± 0.4 May 30
Muan 26.6 ± 2.1 2.7 ± 0.5 3.6 ± 0.3 May 28
Busan 27.2 ± 1.8 2.9 ± 0.3 3.1 ± 0.4 June 01
Boseong 26.9 ± 1.8 2.9 ± 0.2 3.4 ± 0.4 May 26
Bongpyeong 24.9 ± 4.4 2.6 ± 0.3 3.6 ± 0.8 May 27
Seosan 27.6 ± 1.7 2.5 ± 0.3 3.7 ± 0.5 May 28
Taean 26.2 ± 2.0 2.9 ± 0.2 3.3 ±0.3 May 26
Yeojoo 26.1 ± 2.0 2.6 ± 0.1 3.8 ± 0.6 May 28
Yeosu 28.2 ± 1.8 2.9 ± 0.6 3.5 ± 0.6 May 29
Iksan 28.2 ± 3.5 2.8 ± 0.4 3.4 ± 0.3 May 27
Ulsan 29.7 ± 3.3 2.9 ± 0.4 2.9 ± 0.6 May 29
Jangsu 28.8 ± 2.6 3.0 ± 0.4 3.5 ± 0.4 May 29
Chonan 30.0 ± 2.8 2.9 ± 0.4 3.5 ± 0.4 May 30
Hongseon 27.6 ± 3.3 2.9 ± 0.4 3.7 ± 0.5 May 30
Pochun 31.0 ± 1.6 2.2 ± 0.4 4.4 ± 0.9 May 28
Yongin 36.1 ± 2.5 2.0 ± 0.3 3.9 ± 0.6 May 26
Bucheon 32.2 ± 2.2 2.1 ± 0.3 4.2 ± 0.8 May 28
Geumsan 31.1 ± 4.0 2.2 ± 0.3 4.7 ± 0.9 May 25
Data were borrowed from authors' previous report (Kim and Lee, 2007).
Kor. J. Hort. Sci. Technol. 26(1), March 2008 73
얻어 primer당 평균 band수는 3.4개였으며, 20-mers primer
로부터는 90개의 polymorphic band를 얻어 primer당 평균
15개의 band를 얻었다. 10-mers보다 20-mers primer에서
polymorphism이 높았으며, 유연관계 분석결과도 20-mers
primer에서 형태적 특성과 잘 일치하였다. 31개 수집종들은
크게 2군으로 분류할 수 있었으며, I군에는 완주 및 군산 수
집종을 포함한 27개 수집종이 속하였고, 꽃이 피지 않는 완
주와 군산 수집종을 제외한 I군 내의 수집종들 간에는 매우
가까운 유연관계를 보였다. II군에는 줄기와 잎이 가늘고 꽃
이 잘 피는 금산, 부천, 포천, 용인 등 4 수집종이 속하였다.

영어초록

The genetic relationship among 31 Sedum sarmentosum local strains was analyzed using randomly
amplified polymorphic DNA (RAPD) with fifteen 10-mers random primers (Operon Technologies Inc., USA)
and twelve 20-mers random primers (SeouLin Bioscience Inc., Korea). Forty-six polymorphic bands (3.3 per
primer) and ninety (15.0 per primer) were generated by polymerase chain reaction with selected fourteen
10-mers primers, and six 20-mers primers, respectively. The production of polymorphic bands in 20-mers
primers was more efficient than in 10-mers primers. The cluster analysis based on RAPD markers generated
by 20-mers primers was matched well with morphological characteristics compared to 10-mers primer.
Thirty-one local strains analyzed with UPGMA were clustered into 2 groups. Twenty-seven local strains were
clustered into the group Ⅰ, and the genetic relationship in Group I was very closed except Wanju and Gunsan
local strains as no-flowering. Group Ⅱ included Keumsan, Bucheon, Pocheon and Yongin local strains with
long hight, slender stem, narrow leaf, and abundant flowering characteristics.

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