蚯蚓 추출물이 흰쥐의 뇌허혈과 세포에 미치는 효과 (Effects of Lumbricus Extract on Cerebral Ischemia and Cells in Rats)

서지정보

· 발행기관 : 한방재활의학과학회
· 수록지 정보 : 한방재활의학과학회지 / 20권 / 3호 / 1 ~ 11페이지
· 저자명 : 유덕선, 염승룡, 권영달, 송용선

초록

Objectives :This study was designed to investigate the effects of Lumbricus extract(LE) on the regional cerebral blood flow(rCBF) in ischemic rats, further to determine the mechanism of action of LE, and the effects that LE inhibits lactate dehydrogenase(LDH) activity in brain cells.
Methods and materials :This study, ischemic rats were divided into total four group: control group(n=6), experimental group Ⅰ(LE treated group)(n=6), experimental group Ⅱ(LE treated group after pretreatment with indomethacin)(n=6), experimental group Ⅲ(LE treated group after pretreatment with methylene blue)(n=6). And the measurement that LE inhibits LDH activity in the damage to brain cells to N-methyl-D-aspartic acid(NMDA).
The changes of rCBF were determinated by laser-doppler flowmetry(LDF), and LDH activity was determinated by microplate reader in vitro.
Results :1. The rCBF was significantly improved by LE(10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, compared with the control group.
2. The rCBF was significantly increased by LE after pretreatment with indomethacin(1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase, during the period of cerebral reperfusion, compared with the LE group, and rCBF was accelerated by LE after pretreatment with methylene blue(10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase during the period of cerebral reperfusion, compared with the control group.
3. LE significantly inhibited LDH activity in vitro in a dose-dependent manner.
Conclusions :From the above results, these were suggested that Lumbricus had anti-ischemia action in connection with cyclooxygenase and might prevent the brain cells death through inhibited LDH activity.

영어초록

Objectives :This study was designed to investigate the effects of Lumbricus extract(LE) on the regional cerebral blood flow(rCBF) in ischemic rats, further to determine the mechanism of action of LE, and the effects that LE inhibits lactate dehydrogenase(LDH) activity in brain cells.
Methods and materials :This study, ischemic rats were divided into total four group: control group(n=6), experimental group Ⅰ(LE treated group)(n=6), experimental group Ⅱ(LE treated group after pretreatment with indomethacin)(n=6), experimental group Ⅲ(LE treated group after pretreatment with methylene blue)(n=6). And the measurement that LE inhibits LDH activity in the damage to brain cells to N-methyl-D-aspartic acid(NMDA).
The changes of rCBF were determinated by laser-doppler flowmetry(LDF), and LDH activity was determinated by microplate reader in vitro.
Results :1. The rCBF was significantly improved by LE(10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, compared with the control group.
2. The rCBF was significantly increased by LE after pretreatment with indomethacin(1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase, during the period of cerebral reperfusion, compared with the LE group, and rCBF was accelerated by LE after pretreatment with methylene blue(10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase during the period of cerebral reperfusion, compared with the control group.
3. LE significantly inhibited LDH activity in vitro in a dose-dependent manner.
Conclusions :From the above results, these were suggested that Lumbricus had anti-ischemia action in connection with cyclooxygenase and might prevent the brain cells death through inhibited LDH activity.

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