골 재생용 신규 하이드록시아파타이트/폴리락티드 이중구조 다공성 지지체의 구조 및 특성 (Structure and Characteristics of Novel Hydroxyapatite/Polylactide Bi-Layered Porous Scaffold for Bone Regeneration)

서지정보

· 발행기관 : 한국조직공학과 재생의학회
· 수록지 정보 : 조직공학과 재생의학 / 6권 / 13호 / 1241 ~ 1251페이지
· 저자명 : 손준식, 박귀덕, 한동근, Guda Teja, Mark Appleford, Joo L. Ong, 오선호

초록

The goal of this study was to develop novel hydroxyapatite (HAp)/ polylactide (PLA) bi-layered porous
scaffold for tissue-engineered bone regeneration. To mimic normal bone structures, the outside cortical-like shells
fabricated consisted of porous HAp and an inner trabecular-like core was made of porous PLA. Cortical-like porous
HAp scaffolds with hollow in its inside was fabricated using a polymer sponge template. Trabecular-like porous
PLA scaffold was directly formed by a particle leaching/gas foaming technique with sodium percarbonate as a novel
porogen in inner diameter of HA scaffolds. The oxygen plasma treatment was performed to modify the PLA surface
of bi-layered porous scaffold. The plasma-treated bi-layered scaffold was subsequently dipped in an alcoholic solution
containing calcium and phosphate ions to deposit precursors on the surface. The surface modified bi-layered
scaffold was immersed in simulated body fluid (SBF) at 5 times ionic concentration for 24 hr to obtain the final bonelike
apatite coated HAp/PLA bi-layered porous scaffold. It was observed that the HAp and PLA parts of fabricated
bi-layered scaffolds contain open and interconnected pore structures, with no structural delaminations on the external
surfaces. Using helium pycnometry, the maximum porosity was observed to be greater than 96% for bi-layered
scaffold. Additionally, the distribution of pore size for the PLA scaffolds was from 100 to 700 μm. Also, the dense
and uniform bone-like apatite layer was successfully formed on surface of HAp/PLA bi-layered scaffold after
immersion for 1 day in SBF solution. Total protein and alkaline phosphatase (ALP) content of bi-layered scaffold
were higher than the control HAp scaffold during 4 weeks in vitro culture study using human embryonic palatal mesenchymal
(HEPM) cells. It is indicated that bi-layered porous scaffold has stronger ability to induce HEPM cell
attachment, proliferation, differentiation, and mineralization than that of the control HAp porous scaffold. The
resulting apatite coated HAp/PLA bi-layered porous scaffold is more bioactive that might be applicable as a promising
scaffold for osteogenesis.

영어초록

The goal of this study was to develop novel hydroxyapatite (HAp)/ polylactide (PLA) bi-layered porous
scaffold for tissue-engineered bone regeneration. To mimic normal bone structures, the outside cortical-like shells
fabricated consisted of porous HAp and an inner trabecular-like core was made of porous PLA. Cortical-like porous
HAp scaffolds with hollow in its inside was fabricated using a polymer sponge template. Trabecular-like porous
PLA scaffold was directly formed by a particle leaching/gas foaming technique with sodium percarbonate as a novel
porogen in inner diameter of HA scaffolds. The oxygen plasma treatment was performed to modify the PLA surface
of bi-layered porous scaffold. The plasma-treated bi-layered scaffold was subsequently dipped in an alcoholic solution
containing calcium and phosphate ions to deposit precursors on the surface. The surface modified bi-layered
scaffold was immersed in simulated body fluid (SBF) at 5 times ionic concentration for 24 hr to obtain the final bonelike
apatite coated HAp/PLA bi-layered porous scaffold. It was observed that the HAp and PLA parts of fabricated
bi-layered scaffolds contain open and interconnected pore structures, with no structural delaminations on the external
surfaces. Using helium pycnometry, the maximum porosity was observed to be greater than 96% for bi-layered
scaffold. Additionally, the distribution of pore size for the PLA scaffolds was from 100 to 700 μm. Also, the dense
and uniform bone-like apatite layer was successfully formed on surface of HAp/PLA bi-layered scaffold after
immersion for 1 day in SBF solution. Total protein and alkaline phosphatase (ALP) content of bi-layered scaffold
were higher than the control HAp scaffold during 4 weeks in vitro culture study using human embryonic palatal mesenchymal
(HEPM) cells. It is indicated that bi-layered porous scaffold has stronger ability to induce HEPM cell
attachment, proliferation, differentiation, and mineralization than that of the control HAp porous scaffold. The
resulting apatite coated HAp/PLA bi-layered porous scaffold is more bioactive that might be applicable as a promising
scaffold for osteogenesis.

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