선녹콩 개체간 및 개체내 단백질 함량 변이 (Intra- and Inter-Variation of Protein Content in Soybean Cultivar Seonnogkong)

서지정보

· 발행기관 : 한국작물학회
· 수록지 정보 : 한국작물학회지 / 53권 / 78 ~ 83페이지
· 저자명 : 임무혁, 정명근

초록

Soybean [Glycine max (L.)] is a major source
of protein for human and animal feed. Inter- and intragenotype
variation of soybean protein has been investigated
by soybean researchers. However, limited sample amount
of soybean single seed there is no report that investigated
intra-plant variation of soybean protein within soybean plant.
Recently a non-destructive NIR (near-infrared reflectance)
spectroscopy using single seed grain to analyze seed protein
was developed. The objectives of this study were to
understand variation of seed protein content within plant
and to determine the amount of minimum sample size which
can represent protein content for a soybean plant. Frequency
distribution of protein content within plant showed
normal distribution. There was an intra-cultivar variation
for protein content in soybean cultivar Seonnogkong. Difference
of protein content among single plants of Seonnokong
was recognized at 5% level. Seeds in lower position on
plant stem tended to accumulate more protein than in higher
position. There was significant difference for protein content
between sample size 5 seeds and sample size of more than
5 seeds (10, 20, 30, 40, and 50 seeds) at a soybean plant
with 57 seeds however no difference was recognized among
sample size (5, 10, 20, and 30 seeds) at a soybean plant
with 33 seeds. Around 20% seeds of soybean from single
plant needed to determine the protein content to represent
protein content of single soybean plant. This study is the
first one to report evidence of intra-plant variation for protein
content which detected by non-destructive NIR spectroscopy
using single seed grain in soybean.

영어초록

Soybean [Glycine max (L.)] is a major source
of protein for human and animal feed. Inter- and intragenotype
variation of soybean protein has been investigated
by soybean researchers. However, limited sample amount
of soybean single seed there is no report that investigated
intra-plant variation of soybean protein within soybean plant.
Recently a non-destructive NIR (near-infrared reflectance)
spectroscopy using single seed grain to analyze seed protein
was developed. The objectives of this study were to
understand variation of seed protein content within plant
and to determine the amount of minimum sample size which
can represent protein content for a soybean plant. Frequency
distribution of protein content within plant showed
normal distribution. There was an intra-cultivar variation
for protein content in soybean cultivar Seonnogkong. Difference
of protein content among single plants of Seonnokong
was recognized at 5% level. Seeds in lower position on
plant stem tended to accumulate more protein than in higher
position. There was significant difference for protein content
between sample size 5 seeds and sample size of more than
5 seeds (10, 20, 30, 40, and 50 seeds) at a soybean plant
with 57 seeds however no difference was recognized among
sample size (5, 10, 20, and 30 seeds) at a soybean plant
with 33 seeds. Around 20% seeds of soybean from single
plant needed to determine the protein content to represent
protein content of single soybean plant. This study is the
first one to report evidence of intra-plant variation for protein
content which detected by non-destructive NIR spectroscopy
using single seed grain in soybean.

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