L. acidophilus CT 01의 배양 온도의 변화가 저장, 냉장, 열 및 건조에 미치는 영향

서지정보

· 발행기관 : 강원대학교 동물자원공동연구소
· 수록지 정보 : 동물자원연구 / 15권
· 저자명 : 유근형, 권일경, 김거유

목차

ABSTRACT Ⅰ. 서론 Ⅱ. 재료 및 방법 1. 사용균주 2. 생균수 측정 3. 배양 온도 변화에 따른 L. acidophilus CT 01의 생장 4. 저장성 및 냉동내성 5. 열 및 건조에 대한 저항성 6. 통계분석 Ⅲ. 결과 및 고찰 1. 배양 온도 변화에 따른 L. acidophilus CT 01의 생장 2. 배양 온도 변화에 따른 L. acidophilus CT 01의 저장성및 냉동내성 3. 배양 온도 변화에 따른 L. acidophilus CT 01의 열 및건조에 대한 3. 배양 온도 변화에 따른 L. acidophilus CT 01의 열 및 건조에 대한 저항성저항성 Ⅳ. 요약 Ⅴ. 참고문헌

영어초록

This study was carried out to determine the storage, cryotolerance, heat and drying resistance when the L. acidophilus growing at optimal temperature was changed growth temperature and to apply it to probiotics production so as to help intensify the viability of lactic acid bacteria in the products. L. acidophilus CT 01 was changed temperature at 22℃ and 28℃ after incubated at 37℃ for 18hours. The viable cell number increased to 4.9×108 and 5.0×108 cfu/mL, respectively in the changed temperature groups, but decreased in the control, rapidly down to 7.4×105 cfu/mL in 36 hours. L. acidophilus CT 01 was stored at 4℃ for 12 days after changed temperature at 22℃ and 28℃ for 48hours, changed temperature groups had a survival rate of 112% and 94.43%, higher than that of control (p<0.05). When stored at room temperature, survival rate in the control was 10.88%, very low, but changed temperature groups had very high survival rate, 74.43% and 85.62% (p<0.05). The cryotolerance of L. acidophilus CT 01 appeared higher than changed temperature groups was observed at 37℃ for 66hours. L. acidophilus CT 01 was heat treated at 60℃ for 15 minutes and 30 minutes to examine heat resistance after changed temperature, the heat resistance of L. acidophilus CT 01 appeared higher than control was observed changed temperature groups. L. acidophilus CT 01 was inoculated by 30% to the carrier after changed temperature, and dried at 50℃ for 12 hours. The highest survival rate of L. acidophilus CT 01 was observed changed temperature groups.

참고자료

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