Effects of cold stress as a pathogenic factor on fatal disease progression of canine herpesvirus

서지정보

· 발행기관 : 충북대학교 동물의학연구소
· 수록지 정보 : Journal of Biomedical Research / 15권 / 3호
· 저자명 : Hyun-A Lee, Sunhwa Hong, Yun-seong Lee, Dong-Woo Kim, Hye-Young Kwon, Cheol-Yong Lee, Okjin Kim

목차

Introduction
Materials and Methods
Animal Experiment
Monitoring of clinical signs, urine analysis, and blood sampling
Hematological and biochemical analysis
Pathological observation
Virus isolation
Polymerase chain reaction (PCR)
Results
Clinical signs and body weight changes
Blood analysis and urine test
Pathological findings
Virus isolation
PCR analysis
Discussion
Acknowledgements
References

영어초록

Canine herpesvirus (CHV) is a member of the alphaherpesvirus subfamily, which can cause severe hemorrhagic diseases in neonatal pups as well as mild or subclinical respiratory infections in adult dogs. We examined the effects of cold stress on disease progression of CHV, an alphaherpesvirus, in neonatal puppies. Eight puppies were challenged intranasally with CHV suspension and divided into a cold stress treatment group and a hyperthermal group. Four pups were left uninoculated as controls and divided into cold and hyperthermal groups. In the challenged cold treatment group, all pups showed CHV-related disease within 5 days; pathological changes were observed in organs of puppies showing clinical symptoms. Grossly, numerous petechial red foci were scattered throughout lungs, kidneys, livers, and intestines of all CHV-infected puppies exposed to cold stress. Most puppies showed typical clinical signs and macroscopic lesions, and CHV infection was confirmed by isolation of the virus. However, in the challenged hyperthermal group, only one of the pups showed mild symptoms of CHV-induced disease. None of the puppies in the uninoculated group showed abnormal signs, although they were exposed to cold stress. These findings indicate that cold stress can cause rapid disease progression of CHV, an alphaherpesvirus.

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