[화학실험기법1 A+]Exp1labreport_Quenching Study with Absorption and Fluorescence Spectroscopy

목차

1. Abstract
2. Results and Discussion
3. Conclusions (결론)
4. References (참고문헌)

본문내용

Abstract
본 실험에서는 UV-vis spectrophotometer와 Fluorescence spectrometer를 이용하여 Coumarin 1과 Quinine sulfate(QS)의 흡광도와 형광을 측정하고 Coumarin 1의 FY를 구했다. 그리고 NaCl 농도에 따른 QS이 흡광도와 형광을 측정함으로써 quenching을 관찰했다. Coumarin 1의 FY Φ_coumarin=0.5608(오차 23.3%)이었다. Quenching 실험에서는 NaCl의 농도가 증가함에 따라 QS의 형광이 감소하는 것을 확인했다. Stern-Volmer equation을 이용해 계산한 2차 소광 상수 k_q=2.43×10^8 M^(-1) s^(-1)이었고 , Debye-Smoluchowski equation을 이용해 계산한 확산 속도 상수 k_diff=2.78×10^7 M^(-1) s^(-1)이었다.

참고자료

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