[A+] 미생물학/면역학 실험 : 쥐 해부(Mouse dissection) 및 유세포분석(Flow cytometry) 결과 레포트

소개

약학대학 과정의 미생물/면역학 실험 레포트입니다.
(다른 자연과학 미생물/면역학 실험 과목의 과정과 거의 같습니다.)
긴말하지 않겠습니다.
A+ 받았던 결과보고서 입니다. 특히 DIscussion(고찰) 부분은 자신있습니다.
받아보시면 절대 후회하지 않으실겁니다.
실험 결과 사진 및 계산 과정 등이 모두 상세히 기술되어 있습니다.

목차

1. Title

2. Abstract

3. Introduction
Ⅰ. 동물 실험이란?
Ⅱ. 실험동물이란?
Ⅲ. Lymphoid Organs
Ⅳ. Flow Cytometry (유세포 분석)

4. Method

5. Result

6. Discussion

7. References

본문내용

1. Title : Mouse dissection and Flow cytometry

2. Abstract
- Laboratory Mice (Mouse dissection) ; Inbred mice
- Primary/Secondary lymphoid organs
- Flow cytometry ; Compensation, Scatter Plot, Gating
- FITC & PE & PI

3. Introduction
Ⅰ. 동물 실험이란?
1. 정의
교육·시험·연구 및 생물학적 제제(製劑)의 생산 등 과학적 목적을 위하여 실험
동물을 대상으로 실시하는 실험 또는 그 과학적 절차

2. 동물 실험의 역사
Figure 1. 동물 실험의 역사

<중 략>

1. Discussion
제 13-14주차 실습에서는 실험용 동물로 널리 사용되고 있는 mouse를 해부하여 Primary lymphoid organ과 Secondary lymphoid organ의 위치를 파악하고, 이를 분리해내는 dissection을 수행하였다. 이어 분리해낸 lymphoid organ들을 Homogenizer Cell Lysis Tissue Grinder라는 유리 재질의 분쇄기로 파쇄 했고, organ 내의 세포들을 buffer에 짜내어 분리했다. 분리한 thymus와 lymph node는 누리끼리 색이었고 이 때문에 분쇄기에서 세포를 짜낼 때도 buffer의 색이 크게 변하지 않았다. 하지만 spleen은 혈액 성분이 많이 지나가는 lymphoid organ으로, 분리한 spleen의 색은 혈액과 똑같은 붉은 색이었다. Organ을 파쇄 할 때에도 buffer의 색이 붉게 물들 정도였는데 이는 spleen에서 추출된 세포 중 적혈구가 많다는 것을 의미한다.
Flow cytometry의 그래프 해석은 다음과 같다.

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