gene cloning

목차

1. what is gene cloning?
2. Gene cloning step 1 (Gets the desired gene)
3. Gene cloning step 2 (Insert the obtained gene into a vector)
4. Gene cloning step 3 (A vector with a DNA insert is injected into the cell)
5. Gene cloning step 4 (select the correct clone)
6. Reference

본문내용

• what is gene cloning?
A DNA fragment containing the gene is ligated into a vector to form a Techniques for making clones by propagation with recombinant DNA molecules

유전자를 가지고 있는 DNA 단편을 벡터(Vector)에 결합시켜 숙주세포 내에서 재조합 DNA 분자로 증식시켜 클론(Clone)을 만드는 기술
이 기술은 DNA 분자를 아주 정확하고 재현성 있는 방식으로 절단하는 것을 요구

Gene cloning step 1 (Gets the desired gene).
• method is Library screening and PCR(polymerase chain reaction)
• PCR product electrophoresis, purification

참고 자료

Rakesh C. Sharma and Robert T. Schimke, "Preparation of Electro-competent E. coli Using Salt-free Growth Medium", Biotechniques 20, 42-44
Cell biology of the Gene(5th edition) by Bruce Alberts, DENNIS BRAY, Karen Hopkin, Alexander Johnson,)
http://www.chem.uga.edu/DoC/index.cfm
http://blog.naver.com/genetic2012?Redirect=Log&logNo=30002630921
http://biochemistry.yonsei.ac.kr/biochem_workshop/bacterial_transformation.php
http://genetics.hannam.ac.kr/lecture/GELab2002/GELAB23-transforamtion.htm