[동물질병학] Gram staining, Nosal swab, striking 실험보고서

소개글

중앙대학교 동물질병학 실습보고서입니다.
영어로 작성하여 가산점받아 1등한 보고서입니다.

목차

1. Gram staining
2. Microbial contamination measurements
3. Nasal swab & Streaking

본문내용

1. Gram staining

Brief : The preliminary classification of a bacterium as gram positive or gram negative is an essential step in both diagnostic microbiology and clinical medicine.
The Gram stain is generally the first procedure performed in the identification of a bacterium and the results of this differential stain often determine the subsequent identification procedures.
Often Gram stain yields equivocal results particularly with gram positive anaerobic bacteria and anaerobic gram negative bacteria.

Protocols :
1. Prepare the slide glass and blow away the oils with the flame
2. Drop the bacterial broth on the glass
3. Heat fixation with the alcohol lamp flame
4. Apply on Primary staining agent(Crystal violet), 1 min
5. Wash the CV and drop the iodine agent, 1 min
6. Wash with the alcohol
7. Do the Counter staining(Safranin), 1 min
8. Wash with water and drop the mineral oil
9. Observe the results of staining with microscope

Result :
Our sample has 2 kinds of bacteria. Purple coccus, and pink bacillcus

참고 자료

standardized Gram staining procedure, D. Claus, World Journal of Microbiology and Biotechnology Volume 8, Number 4 451-452 0959-3993 SCI(E)
해부 병태생리로 이해하는 SIM 통합내과학 2 : 감염, 2013. 4. 15.
Rapid Method That Aids in Distinguishing Gram-Positive from Gram-Negative Anaerobic Bacteria, SHUSHAN HALEBIAN,' BETTY HARRIS,' SYDNEY M. FINEGOLD,",2 AND RIAL D. ROLFEI 2* Medical and Research Services, Veterans Administration Wadsworth Medical Center, Los Angeles, California 900731*, and Department ofMedicine, UCLA Center for the Health Sciences, Los Angeles, California 900242, JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1981, p. 444-448
Vancomycin sensitivity and koh string test as an alternative to gram staining of bacteria, Arthi KAppalaraju B, Parvathi S, Indian Journal of Medical Microbiology Vol. 21 No. 2 121p ~ 123p 0255-085
http://vlab.amrita.edu/?sub=3&brch=73&sim=208&cnt=1
Gram-typing of mastitis bacteria in milk samples using flow cytometry, Langerhuus S.N, Ingvartsen K.L, Bennedsgaard T.W, Røntved C.M, Journal of Dairy Science Vol. 96 No. 1 267p ~ 277p 0022-0302 SCOPUS
Pouring agar plates and streaking or spreading to isolate individual colonies, Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: [e-mail], Methods Enzymol. 2013;533:3-14.
P. M. Rhodes and P. F. Stanbury, Applied Microbial Physiology(1997), IRL PRESS
Robert W. Bauman, Microbiology(2006), PEARSON