분자생물학기초실험/ 분자생명공학기초실험 9주차: Protein Purification [A+ 리포트]

소개

연세대학교 2016년 2학기 A+ 받은 리포트입니다.
실험관련 topic에 대해 많은 조사를 했기에 이 자료 하나로 충분할거에요.

목차

1. Objective
2. Experimental Background
3. Method
4. Results/Discussion
5. Quiz
6. References

본문내용

<Experimental Background>
Protein purification is a technique used to isolate a single protein type that is usually extracted from a sample of biological tissue or complex mixture such as a cell lysate. Many times, single specific protein interest is caught in a matrix of other protein or even non-protein parts and hence protein purification must be done to separate the single desired proteins from all other mixtures.

<Results/Discussion>
AKTA FPLC purification of His-tagged protein was performed and following curves were attained after applying the samples through immobilized Ni-columns. 10mM binding buffer & 500mM elution buffer (with imidazole) were used.

참고자료

· Basic Molecular Biotechnology Laboratory Booklet – Department of Biotech.Yonsei University.
· Cable, By Jennifer, By Alex Cohen, Jennifer Aniston, Iqra Qaddir, D., Rodrigo, Jennifer Cable, Gabriel, Martin, MarkB, Max-ch, and Jeff Hollins. "How to Design the Perfect Protein Purification Buffer - Bitesize Bio." Bitesize Bio. Bitesize Bio, 27 Feb. 2014. Web. 20 Nov. 2016. <http://bitesizebio.com/7893/how-to-design-the-perfect-protein-purificationbuffer/>.
· "Guidelines for Protein Purification at Low Temperature." GE Healthcare Life Sciences. General Electric Company, Aug. 2015. Web. 20 Nov. 2016. <http://www.gelifesciences.com/>.
· "Imidazole." ACS Reagent, ≥99% (titration) | C3H4N2 | Sigma-Aldrich. Web. 22 Nov. 2016. <http://www.sigmaaldrich.com/catalog/product/sial/i2399?lang=ko® ion=KR>.
· Man, Protein. "What Are Protease Inhibitors and How Do They Work?" What Are Protease Inhibitors and How Do They Work? Web. 20 Nov. 2016. <http://info.gbiosciences.com/blog/bid/160009/What-are-Protease-Inhibitorsand-How-Do-They-Work>.