[생화학실험] BSA 단백질 추출 실험레포트
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* 본 문서는 한글 2005 이상 버전에서 작성된 문서입니다.
한글 2002 이하 프로그램에서는 열어볼 수 없으니, 한글 뷰어프로그램(한글 2005 이상)을 설치하신 후 확인해주시기 바랍니다.
소개글
생화학실험 BSA 단백질 추출에 관한 실험레포트입니다.
영문으로 작성되어있습니다.
목차
Section1. Introduction
Section2. Materials
Section3. Procedure
1) Plan& prepare
2) CM
3) Affinity chromatography
4) Analysis
5) Strategy & Metho
Section4. Result
1) SDS-PAGE results
2) Purification table
3) Chromatography results
Section5. Discussion
Section6. Reference
본문내용
1. Introduction
Extraction of protein is performed in many experiments. Extraction of protein that we want is a very important process in all experiment. using chromatography, we had to extract BSA in sample that has various protein through this time experiment.
We used CM, DEAE and Affinity chromatography. Therefore, We should know each principle of some chromatography in this experiment. So, after I know these principles, I used these principles for extraction of protein. And we had to use proper buffer and method for extraction of BSA which has good yield and good purity.
2. Materials
- sample
10% Albumin + 90% B.P
- materials for Ion-exchange chromatography
column peripherals(pumps, UV monitor, fraction collector, detector, chart recorder)
CM column and etc.
- materials for affinity chromatography
column peripherals(pumps, UV monitor, fraction collector, detector, chart recorder)
HiTrap Blue HP column and etc.
- materials for SDS-PAGE
power supplier, Gel box, plates, comb, spacer, running buffer
참고 자료
http://biotech.about.com/od/protocols/a/ProteinPurify.htm
http://blog.naver.com/lovehrh?Redirect=Log&logNo=30020108297
http://blog.naver.com/williamaz2?Redirect=Log&logNo=60018113291
Experiments in biochemistry
Lehninger principles of Biochemistry