[생화학실험] BSA 단백질 추출 실험레포트

소개

생화학실험 BSA 단백질 추출에 관한 실험레포트입니다.
영문으로 작성되어있습니다.

목차

Section1. Introduction

Section2. Materials

Section3. Procedure
1) Plan& prepare
2) CM
3) Affinity chromatography
4) Analysis
5) Strategy & Metho

Section4. Result
1) SDS-PAGE results
2) Purification table
3) Chromatography results

Section5. Discussion

Section6. Reference

본문내용

1. Introduction
Extraction of protein is performed in many experiments. Extraction of protein that we want is a very important process in all experiment. using chromatography, we had to extract BSA in sample that has various protein through this time experiment.
We used CM, DEAE and Affinity chromatography. Therefore, We should know each principle of some chromatography in this experiment. So, after I know these principles, I used these principles for extraction of protein. And we had to use proper buffer and method for extraction of BSA which has good yield and good purity.

2. Materials
- sample
10% Albumin + 90% B.P
- materials for Ion-exchange chromatography
column peripherals(pumps, UV monitor, fraction collector, detector, chart recorder)
CM column and etc.
- materials for affinity chromatography
column peripherals(pumps, UV monitor, fraction collector, detector, chart recorder)
HiTrap Blue HP column and etc.
- materials for SDS-PAGE
power supplier, Gel box, plates, comb, spacer, running buffer

참고자료

· http://biotech.about.com/od/protocols/a/ProteinPurify.htm
· http://blog.naver.com/lovehrh?Redirect=Log&logNo=30020108297
· http://blog.naver.com/williamaz2?Redirect=Log&logNo=60018113291
· Experiments in biochemistry
· Lehninger principles of Biochemistry