고려대학교 미생물학 실험 7 Fermentation of Carbohydrates 결과
- 최초 등록일
- 2015.02.20
- 최종 저작일
- 2010.11
- 5페이지/
한컴오피스
- 가격 1,000원
목차
I. Material
II. Method
III. Results
IV. Discussion
V. 출처
본문내용
I. Material
- Strains : Escherichia coli, Enterobacter sp., P. aeruginosa, Bacillus subtilis
- LB broth(+ 0.5% carbohydrates)
- MR-VP broth (/L)
Peptone ------- 7g
Glucose ------- 5g
- Cap tube, Durham tube, Loop, Alcohol ramp, Petri dish, 핀셋, shaking incubator, Micropipette
- Methyl Red : 0.1g의 methyl red를 95% ethanol에 용해 후 200ml D.W.를 첨가한다.
- VP reagent : Absolute alcohol 100mL에 alpha-naphthol 50g을 녹인다.
- Phenol Red
- Barritt's reagents
solution A : 5 g의 α-naphthol을 100mL의 absolute ethanol에 녹인다.
solution B : 40 % KOH
참고 자료
“MR-VP Tests.” Microbugz. Austin Community College. 11 Nov 2010. <http://www.austincc.edu/microbugz/mrvp_test.php>.
Sherwood, Linda, Joanne Willey, and Christopher Woolverton. Prescott, Harley, and Klein's Microbiology. 7th ed. New York: McGraw-Hill, 2008.
“Voges-Proskauer Test.” Food Microbiology. Department of Vet. Microbiology, KVL. 11 Nov 2010. <http://www.microbiologyatlas.kvl.dk/ biologi/english/showbio.asp?articleid=14>.
Wikipedia contributors. "Methyl red." Wikipedia, The Free Encyclopedia. Wikipedia, The Free Encyclopedia, 22 Sep. 2010. Web. 11 Nov. 2010.
