24개월간 자일리톨껌 저작에 따른 S. mutans의 치아우식증 발생과 관련된 독성 유전자 발현 억제효과 (The Inhibitive effect of 24 months xylitol gum chewing on the virulence related gene expression of Streptococcus mutans)

서지정보

· 발행기관 : 대한예방치과·구강보건학회
· 수록지 정보 : 대한구강보건학회지 / 33권 / 4호 / 534 ~ 543페이지
· 저자명 : 김지혜, 서영규, 이영은, 안상헌, 구효진, 최연희, 송근배

초록

Objective: The aim of this study was to examine the effect of chewing gum containing xylitol on inhibiting the virulence related gene expression of Streptococcus mutans (S. mutans).
Methods: The subjects voluntarily participating in a women’s oral health prevention program were divided into two groups (a control and a xylitol group), and then they were followed for 2 years. At baseline, 6, 12, 18 and 24 months whole stimulated saliva samples were collected from the subjects. The analysis of the colony count, stereomicroscopy and real-time reverse transcription polymerase chain reaction were performed to analyze the characteristics of S. mutans.
Results: The S. mutans counts decreased steadily in the xylitol group over the study period (p< 0.05). In addition, the size of the colonies isolated from the xylitol group was smaller and their rough surface changed into a smooth surface. The expression of gtfA, gtfB, gtfC, gtfD, ftf, Putative inorganic ion ABC transporter and ATP-binding protein gene were significantly reduced in the xylitol group (p<0.05). The expression of gtfA, gtfB, gtfD, ftf, Putative inorganic ion ABC transporter and the ATP-binding protein gene in the xylitol group were significantly lower than those of the control group at 6, 12, 18 and 24
months (p< 0.05).
Conclusions: These findings indicate that chewing xylitol gum over a long period may decrease the expression of the genes associated with S. mutans virulence, which can result in the reduced size and growth of S. mutans colonies.

영어초록

Objective: The aim of this study was to examine the effect of chewing gum containing xylitol on inhibiting the virulence related gene expression of Streptococcus mutans (S. mutans).
Methods: The subjects voluntarily participating in a women’s oral health prevention program were divided into two groups (a control and a xylitol group), and then they were followed for 2 years. At baseline, 6, 12, 18 and 24 months whole stimulated saliva samples were collected from the subjects. The analysis of the colony count, stereomicroscopy and real-time reverse transcription polymerase chain reaction were performed to analyze the characteristics of S. mutans.
Results: The S. mutans counts decreased steadily in the xylitol group over the study period (p< 0.05). In addition, the size of the colonies isolated from the xylitol group was smaller and their rough surface changed into a smooth surface. The expression of gtfA, gtfB, gtfC, gtfD, ftf, Putative inorganic ion ABC transporter and ATP-binding protein gene were significantly reduced in the xylitol group (p<0.05). The expression of gtfA, gtfB, gtfD, ftf, Putative inorganic ion ABC transporter and the ATP-binding protein gene in the xylitol group were significantly lower than those of the control group at 6, 12, 18 and 24
months (p< 0.05).
Conclusions: These findings indicate that chewing xylitol gum over a long period may decrease the expression of the genes associated with S. mutans virulence, which can result in the reduced size and growth of S. mutans colonies.

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