고래회충 유충의 충체 및 분비 배설 단백질의가수분해효소 활성 분석 (Hydrolases Activity of Excretory-Secretory Proteins and Crude Extract from Anisakis simplex Larval Stages)

서지정보

· 발행기관 : 고신대학교(의대) 고신대학교 의과대학 학술지
· 수록지 정보 : 고신대학교 의과대학 학술지 / 23권 / 4호 / 64 ~ 68페이지
· 저자명 : 옥미선

초록

Background and Objectives : Anisakis simplex L3 (third-stage larva) is the most prevalent species causing anisakidosis which is associated with consumption of raw or insufficiently processed fish or cephalopods. Adult worm of A. simplex inhabits in the stomach of cetaceans and pinnipeds such as whales, porpoises, and seals. Eggs, passed in the feces of the adult worm in marine mammals, hatch to liberate larvae, which then can be ingested by marine crustaceans. The third-stage larvae in the crustaceans are eaten by various species of fish or cephalopods of paratenic or transport hosts.
Anisakidosis can cause not only direct tissue damage but also severe allergic response caused by larvae as well as by excretory-secretory (ES) products. The objectives of this study was to check the pattern of hydrolase activity from extracts of L3, L4 stages and ES products of A. simplex larvae.
s Materials and Methods : A. simplex L3 were collected from mackerels ( Scomber japonicus ). The third and fourth-stage larval extracts were obtained by homogenising and centrifugation. After A. simplex L3 were kept in PBS for 9 consecutive days, the supernatant was used as the excretory-secretory products. To identify the hydrolase activity in larval extract and ES products, SDS-PAGE and API ZYM method were carried out.
s Results : Six common positive enzymes were detected in L3, L4 and ES products belonging to four general groups as follows: (1) phosphatase group: alkaline phosphatase, acid phosphatase and naphthol-AS-BI-phosphohydrolase; (2) esterase group: esterase lipase (C8); (3) protease group: leucine arylamidase; and (4) glucosidase group: -glucosidase. α ES products was relatively rich in trypsin and -chymotrypsin, apart from the common positive hydrolases. α s Conclusions : A. simplex L3, L4 extract and ES products contain several kinds of hydrolases of phosphatase, esterase, protease and glucosidase group, which appear to be important in larval development and host -parasite interaction

영어초록

Background and Objectives : Anisakis simplex L3 (third-stage larva) is the most prevalent species causing anisakidosis which is associated with consumption of raw or insufficiently processed fish or cephalopods. Adult worm of A. simplex inhabits in the stomach of cetaceans and pinnipeds such as whales, porpoises, and seals. Eggs, passed in the feces of the adult worm in marine mammals, hatch to liberate larvae, which then can be ingested by marine crustaceans. The third-stage larvae in the crustaceans are eaten by various species of fish or cephalopods of paratenic or transport hosts.
Anisakidosis can cause not only direct tissue damage but also severe allergic response caused by larvae as well as by excretory-secretory (ES) products. The objectives of this study was to check the pattern of hydrolase activity from extracts of L3, L4 stages and ES products of A. simplex larvae.
s Materials and Methods : A. simplex L3 were collected from mackerels ( Scomber japonicus ). The third and fourth-stage larval extracts were obtained by homogenising and centrifugation. After A. simplex L3 were kept in PBS for 9 consecutive days, the supernatant was used as the excretory-secretory products. To identify the hydrolase activity in larval extract and ES products, SDS-PAGE and API ZYM method were carried out.
s Results : Six common positive enzymes were detected in L3, L4 and ES products belonging to four general groups as follows: (1) phosphatase group: alkaline phosphatase, acid phosphatase and naphthol-AS-BI-phosphohydrolase; (2) esterase group: esterase lipase (C8); (3) protease group: leucine arylamidase; and (4) glucosidase group: -glucosidase. α ES products was relatively rich in trypsin and -chymotrypsin, apart from the common positive hydrolases. α s Conclusions : A. simplex L3, L4 extract and ES products contain several kinds of hydrolases of phosphatase, esterase, protease and glucosidase group, which appear to be important in larval development and host -parasite interaction

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