배양된 쥐 대뇌피질 신경세포에서 산소 결핍에 의한 허혈성 손상에 대한 항산화제 quercetin의 보호 효과 (Neuroprotective Effect of Quercetin against Damage Induced by Oxygen Deprivation in Cultured Rat Cortical Neurons)

서지정보

· 발행기관 : 대한응급의학회
· 수록지 정보 : 대한응급의학회지 / 20권 / 2호 / 170 ~ 176페이지
· 저자명 : 김훈, 이석우, 박종일, 박정수

초록

Purpose: Neuronal hypoxia is clinically related to stroke,
cardiac arrest, asphyxia, and various cases involving metabolic
encephalopathy, which has led to much research looking
for new neuroprotective strategies. Quercetin belongs to
an extensive class of polyphenolic flavonoid compounds,
which are almost ubiquitous in plants and plant food
sources. Quercetin is known as a strong free radical scavenger
and an inhibitor of the production of reactive oxygen
species (ROS). However, the effects of quercetin on hypoxia-
induced oxidative cell damage have rarely been investigated.
This report describes studies in cultured rat cortical
neurons on the cellular effects of quercetin on neural damage
induced by oxygen-deprivation.
Methods: The experiment was conducted in vitro using primary
culture of rat cortical neurons. Hypoxia induction was
performed with a hypoxic chamber. The free radical scavenging
activity of quercetin was assayed in cell-free systems
using a stable free radical, 1,1-diphenyl-2-picrylhydrazyl
(DPPH). Cytotoxicity was studied with the MTT
method. The optical density of the neurons was measured
at 540 nm using an ELISA reader. We used an inverted
microscope to examine the morphological changes that followed
hypoxia induction and quercetin treatment.
Results: An anti-DPPH radical assay showed that
quercetin inhibited the production of DPPH radicals in vitro
and that its radical scavenging activity was superior to
ascorbic acid and n-acetylcysteine. According to the MTT
assay, incubation of cortical neurons with quercetin protected
the neurons from hypoxia-induced cytotoxicity.
Conclusions: The results of this study suggest that at a
low concentration (100 nM) quercetin has a neuroprotective
effect on hypoxia-induced neuronal cell death; at a high
concentration (100 uM) the protective effects for hypoxiainduced
neuronal damage were reduced because of
quercetin’s apoptotic effects.

영어초록

Purpose: Neuronal hypoxia is clinically related to stroke,
cardiac arrest, asphyxia, and various cases involving metabolic
encephalopathy, which has led to much research looking
for new neuroprotective strategies. Quercetin belongs to
an extensive class of polyphenolic flavonoid compounds,
which are almost ubiquitous in plants and plant food
sources. Quercetin is known as a strong free radical scavenger
and an inhibitor of the production of reactive oxygen
species (ROS). However, the effects of quercetin on hypoxia-
induced oxidative cell damage have rarely been investigated.
This report describes studies in cultured rat cortical
neurons on the cellular effects of quercetin on neural damage
induced by oxygen-deprivation.
Methods: The experiment was conducted in vitro using primary
culture of rat cortical neurons. Hypoxia induction was
performed with a hypoxic chamber. The free radical scavenging
activity of quercetin was assayed in cell-free systems
using a stable free radical, 1,1-diphenyl-2-picrylhydrazyl
(DPPH). Cytotoxicity was studied with the MTT
method. The optical density of the neurons was measured
at 540 nm using an ELISA reader. We used an inverted
microscope to examine the morphological changes that followed
hypoxia induction and quercetin treatment.
Results: An anti-DPPH radical assay showed that
quercetin inhibited the production of DPPH radicals in vitro
and that its radical scavenging activity was superior to
ascorbic acid and n-acetylcysteine. According to the MTT
assay, incubation of cortical neurons with quercetin protected
the neurons from hypoxia-induced cytotoxicity.
Conclusions: The results of this study suggest that at a
low concentration (100 nM) quercetin has a neuroprotective
effect on hypoxia-induced neuronal cell death; at a high
concentration (100 uM) the protective effects for hypoxiainduced
neuronal damage were reduced because of
quercetin’s apoptotic effects.

참고자료

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