인간 폐암 세포주에서 Widdrol에 의한 Apoptosis 유도 (Apoptosis Induced by Widdrol in Human Lung Carcinoma Cells)

서지정보

· 발행기관 : 대한암예방학회
· 수록지 정보 : 대한암예방학회지 / 14권 / 3호 / 256 ~ 273페이지
· 저자명 : 오유나, 홍윤경, 윤희정, 이은우, 최영현, 권현주, 김병우, 이현태, 박철

초록

Widdrol, a crystalline, natural substance, is an odorant derivative contained in various plants such as
the Juniperus sp., including Juniperus chinensis. Though widdrol has been known to possess anti-fungal
activity against Botrytis cinerea, anti-cancer properties of widdrol are not well understood. Therefore, we
report the cytotoxic activity and the mechanism of cell death exhibited by the widdrol from Juniperus
chinensis against human lung carcinoma A549 cells. Treatment A549 cells with various concentrations
of widdrol resulted in growth inhibition and apoptosis induction in a dose-dependent manner as determined
by cell viability, chromatin condensation, and DNA fragmentation analysis. The Western blot analysis
revealed that apoptosis in A549 cells was associated with release of cytochrome c from mitochondria
into the cytosol, activation of caspase-3, -8, -9 and proteolytic cleavage of poly (ADP-ribose) polymerase
(PARP). These results suggest that widdrol exhibits antiproliferative effect on A549 cells via apoptosis,
and can be a potential candidate in the field of anticancer drug discovery. (Cancer Prev Res 14, 256-264,
2009)

영어초록

Widdrol, a crystalline, natural substance, is an odorant derivative contained in various plants such as
the Juniperus sp., including Juniperus chinensis. Though widdrol has been known to possess anti-fungal
activity against Botrytis cinerea, anti-cancer properties of widdrol are not well understood. Therefore, we
report the cytotoxic activity and the mechanism of cell death exhibited by the widdrol from Juniperus
chinensis against human lung carcinoma A549 cells. Treatment A549 cells with various concentrations
of widdrol resulted in growth inhibition and apoptosis induction in a dose-dependent manner as determined
by cell viability, chromatin condensation, and DNA fragmentation analysis. The Western blot analysis
revealed that apoptosis in A549 cells was associated with release of cytochrome c from mitochondria
into the cytosol, activation of caspase-3, -8, -9 and proteolytic cleavage of poly (ADP-ribose) polymerase
(PARP). These results suggest that widdrol exhibits antiproliferative effect on A549 cells via apoptosis,
and can be a potential candidate in the field of anticancer drug discovery. (Cancer Prev Res 14, 256-264,
2009)

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