울금(鬱金) 추출물의 미백 활성 및 진피 섬유구조 단백질에 미치는 효과 (Effect of Curcuma longa L. Extract on the Melanin Accumulation and Expression of Skin Fibril Proteins)

서지정보

· 발행기관 : 대한본초학회
· 수록지 정보 : 대한본초학회지 / 34권 / 2호 / 75 ~ 82페이지
· 저자명 : 김혜옥

초록

Objectives : In this study, various biological effects of Curcuma longa L. have been studied, however, beneficial effect of Curcuma longa L. in skin health remain still unclear. In this study, Curcuma longa L. water extract (CLE) was prepared. Inhibitory effect of CLE on melanin accumulation of B16F10 cells and expression levels of skin fibril-related proteins of human skin fibroblasts (HSF) were evaluated.
Methods : The cytotoxic effect of CLE in B16F10 cells and HSF were examined by MTT assay. Inhibitory effect of CLE on the -MSH- and IBMX-induced melanin accumulation and tyrosinase activity were evaluated in B16F10 cells. The expression levels of connective tissue growth factor (CCN2), Smad2, procollagen 12, collagen 12, and fibronectin in CLE-treated HSF were analyzed by western blotting.
Results : The CLE treatment (concentrations 10 to 400 g/ml) for 72 h did not affect to the B16F10 viability. However, 200 and 400 g/ml of CLE treatment for 24 h showed cytotoxic effect in HSF. Therefore, the concentrations 10, 50, and 100 g/ml of CLE were chosen in this study. The CLE treatment for 72 h dose dependently and significantly suppressed melanin accumulation and tyrosinase activity of B16F10 cells. In addition, the CLE treatment up-regulated expression levels of skin fibril-related proteins such as CCN2, Smad2, procollagen 12, collagen 12, and fibronectin.
Conclusions : In conclusion, these results suggest that the CLE could be used as a natural material for skin health.

영어초록

Objectives : In this study, various biological effects of Curcuma longa L. have been studied, however, beneficial effect of Curcuma longa L. in skin health remain still unclear. In this study, Curcuma longa L. water extract (CLE) was prepared. Inhibitory effect of CLE on melanin accumulation of B16F10 cells and expression levels of skin fibril-related proteins of human skin fibroblasts (HSF) were evaluated.
Methods : The cytotoxic effect of CLE in B16F10 cells and HSF were examined by MTT assay. Inhibitory effect of CLE on the -MSH- and IBMX-induced melanin accumulation and tyrosinase activity were evaluated in B16F10 cells. The expression levels of connective tissue growth factor (CCN2), Smad2, procollagen 12, collagen 12, and fibronectin in CLE-treated HSF were analyzed by western blotting.
Results : The CLE treatment (concentrations 10 to 400 g/ml) for 72 h did not affect to the B16F10 viability. However, 200 and 400 g/ml of CLE treatment for 24 h showed cytotoxic effect in HSF. Therefore, the concentrations 10, 50, and 100 g/ml of CLE were chosen in this study. The CLE treatment for 72 h dose dependently and significantly suppressed melanin accumulation and tyrosinase activity of B16F10 cells. In addition, the CLE treatment up-regulated expression levels of skin fibril-related proteins such as CCN2, Smad2, procollagen 12, collagen 12, and fibronectin.
Conclusions : In conclusion, these results suggest that the CLE could be used as a natural material for skin health.

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