발효 황련해독탕의 생물 전환 성분분석 (Analysis of Bioconversion Components of Fermentation Hwangryunhaedok-tang)

서지정보

· 발행기관 : 대한약학회
· 수록지 정보 : 약 학 회 지 / 57권 / 4호 / 293 ~ 298페이지
· 저자명 : 이광진, 이보형, 정필문, 량춘, 마진열

초록

Hwangryunhaedok-tang (HRT) is a traditional herbal medicine, which has been known as a useful prescriptionfor anti-biotic, anti-inflammatory, anti-oxidative and immunosuppressive activity. In this study, the variation in the amountof eight bioactive components of Hwangryunhaedok-tang (HRT) and its fermentation HRT with Lactobacillus casei KFRI127, Lactobacillus curvatus KFRI 166 and Lactobacillus confuses KFRI 227 was investigated via high-performance liquidchromatography coupled with diode array detection (HPLC-DAD). Simultaneous qualitative and quantitative analysis ofeight bioactive components; geniposide, genipin, baicalin, wogonoside, palmatine, berberine, baicalein and wogonin wasachieved by comparing their retention times (tR) and UV spectra with those of the standard components. All calibrationcurve of standard components showed good linearity (r2>0.979). As a result, the geniposide amount was 15.52±0.19 μg/mg that as a main components in HRT. The wogonoside was decreased by 29.28~58.35% with Lactobacillus casei KFRI 127and L. confuses KFRI 227 (3.17±0.31 μg/mg and 3.55±0.13 μg/mg) compared with the original HRT (5.02±0.14 μg/mg).
Otherwise wogonin was increased by 16.28~41.86% with Lactobacillus casei KFRI 127 and L. confuses KFRI 227(0.61±0.01 μg/mg and 0.50±0.02 μg/mg) compared with the original HRT (0.43±0.00 μg/mg). HRT fermented with L. caseiKFRI 127 and L. confuses KFRI 227 were evaluated as creating the changes in wogonoside to that aglycon wogonine. Inthe fermented HRT using Lactobacillus acidophilus KFRI 166, the genipin was only detected, among 3 species of fermentationstrains. Thus, these results considered that the strains 166 were exhibited the remarkable changes in genipin.

영어초록

Hwangryunhaedok-tang (HRT) is a traditional herbal medicine, which has been known as a useful prescriptionfor anti-biotic, anti-inflammatory, anti-oxidative and immunosuppressive activity. In this study, the variation in the amountof eight bioactive components of Hwangryunhaedok-tang (HRT) and its fermentation HRT with Lactobacillus casei KFRI127, Lactobacillus curvatus KFRI 166 and Lactobacillus confuses KFRI 227 was investigated via high-performance liquidchromatography coupled with diode array detection (HPLC-DAD). Simultaneous qualitative and quantitative analysis ofeight bioactive components; geniposide, genipin, baicalin, wogonoside, palmatine, berberine, baicalein and wogonin wasachieved by comparing their retention times (tR) and UV spectra with those of the standard components. All calibrationcurve of standard components showed good linearity (r2>0.979). As a result, the geniposide amount was 15.52±0.19 μg/mg that as a main components in HRT. The wogonoside was decreased by 29.28~58.35% with Lactobacillus casei KFRI 127and L. confuses KFRI 227 (3.17±0.31 μg/mg and 3.55±0.13 μg/mg) compared with the original HRT (5.02±0.14 μg/mg).
Otherwise wogonin was increased by 16.28~41.86% with Lactobacillus casei KFRI 127 and L. confuses KFRI 227(0.61±0.01 μg/mg and 0.50±0.02 μg/mg) compared with the original HRT (0.43±0.00 μg/mg). HRT fermented with L. caseiKFRI 127 and L. confuses KFRI 227 were evaluated as creating the changes in wogonoside to that aglycon wogonine. Inthe fermented HRT using Lactobacillus acidophilus KFRI 166, the genipin was only detected, among 3 species of fermentationstrains. Thus, these results considered that the strains 166 were exhibited the remarkable changes in genipin.

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