熱多寒少湯 煎湯液이 XO/HX에 의해 損傷된 培養脊髓感覺神經細胞 미치는 效果 (Effects of Yuldahansotang water extract on Cultured Spinal Sensory Neurons Damaged by Xanthine Oxidase/Hypoxanthine)

서지정보

· 발행기관 : 사상체질면역의학회
· 수록지 정보 : 사상체질면역의학회지 / 13권 / 1호 / 88 ~ 96페이지
· 저자명 : 홍정아, 김경요, 류도곤, 박혜선, 김형순

초록

To evaluate the effect of Yuldahansotang(YHT) water extract on cultuted mouse spinal sensory neuron which was inhibited by xanthine oxidase(XO) and hypoxanthine(HX)-induced oxigen radicals, MIT assay, NR assay, Neurofilament enzymeimmuno assay and LDH activity assay were carried our after the cultured mouse spinal sensory neuron were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of XO/HX.
The results obtained were as follows: 1. XO/HX, a oxigen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cells on NR assay and MTT assay.
2. MTT50 value and NR50 value pf XO/HX were 20 mU/ml XO/0.2 mM HX and 40 mU/ml XO/0.2 mM HX.
3. YHT water extract have efficacy of increasing neurofilament.
4. YHT water extract have efficacy of increasing LDH activity.
From above the results, It is concluded that YHT has marked efficacy as a treatment for the damages caused in the XO/HX-mediated oxidative process.

영어초록

To evaluate the effect of Yuldahansotang(YHT) water extract on cultuted mouse spinal sensory neuron which was inhibited by xanthine oxidase(XO) and hypoxanthine(HX)-induced oxigen radicals, MIT assay, NR assay, Neurofilament enzymeimmuno assay and LDH activity assay were carried our after the cultured mouse spinal sensory neuron were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of XO/HX.
The results obtained were as follows: 1. XO/HX, a oxigen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cells on NR assay and MTT assay.
2. MTT50 value and NR50 value pf XO/HX were 20 mU/ml XO/0.2 mM HX and 40 mU/ml XO/0.2 mM HX.
3. YHT water extract have efficacy of increasing neurofilament.
4. YHT water extract have efficacy of increasing LDH activity.
From above the results, It is concluded that YHT has marked efficacy as a treatment for the damages caused in the XO/HX-mediated oxidative process.

참고자료

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