양파 노균병균 Peronospora destructor의 분자계통학적 유연관계 분석과 PCR 검출기술 개발 (Phylogenetic Analysis of Downy Mildew Caused by Peronospora destructor and a Method of Detection by PCR)

서지정보

· 발행기관 : 한국균학회
· 수록지 정보 : 한국균학회지 / 45권 / 4호 / 386 ~ 393페이지
· 저자명 : 백창기, 황선경, 박미정, 권영석, 정희영, 박종한

초록

Onion downy mildew, caused by Peronospora destructor, is a major disease in onion cultivation areas in Korea. The causal fungi were collected and analyzed based on sequence similarity and molecular phylogenetic relationships of multi-gene sequences, including the internal transcribed spacer (ITS) region. All isolates from Changnyeong-gun, Hamyang-gun, and Hapcheon-gun in Gyeongnam province, and Muan-gun, Haenam-gun, and Sinan-gun in Jeonnam province were identical in the four types of gene sequences, indicating they were genetically the same strains. In this study, a PCR method was developed based on the ITS gene sequences to amplify the specific DNA fragment for P. destructor only. The detection limit of was total genomic DNA of the P. destructor and the plant 0.7 ng/µL. Therefore, the developed PCR method could be used to detect P. destructor effectively from symptomless onion leaves.

영어초록

Onion downy mildew, caused by Peronospora destructor, is a major disease in onion cultivation areas in Korea. The causal fungi were collected and analyzed based on sequence similarity and molecular phylogenetic relationships of multi-gene sequences, including the internal transcribed spacer (ITS) region. All isolates from Changnyeong-gun, Hamyang-gun, and Hapcheon-gun in Gyeongnam province, and Muan-gun, Haenam-gun, and Sinan-gun in Jeonnam province were identical in the four types of gene sequences, indicating they were genetically the same strains. In this study, a PCR method was developed based on the ITS gene sequences to amplify the specific DNA fragment for P. destructor only. The detection limit of was total genomic DNA of the P. destructor and the plant 0.7 ng/µL. Therefore, the developed PCR method could be used to detect P. destructor effectively from symptomless onion leaves.

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