갑상선암 환자의 말초혈액 백혈구로부터 수지상세포의 유도분화 (Differentiation Induction of Dendritic Cells for Thyroid Cancer Patients)

서지정보

· 발행기관 : 고신대학교(의대) 고신대학교 의과대학 학술지
· 수록지 정보 : 고신대학교 의과대학 학술지 / 21권 / 1호 / 218 ~ 226페이지
· 저자명 : 이대희

초록

Background : Recent studies suggest that immunization with autologous dendritic cells(DCs) result in protective immunity and rejection of established tumors in various human malignancies, It has been reported that a dense infiltration of dendritic cells correlates with a favorable prognosis in several types of cancer. The purpose of this study is to determine whether DCs are generated from peripheral blood monocytes by using cytokines such as Fit-3 ligand， granulocyte macrophage-colony stimulating factor (GM-CSF), IL-4, and TNF- a，and whether cytotoxic T cells activated against the medullary thyroid carcinoma(MTC) tissues by the DCs.


Methods : Peripheral blood was obtained from 2 patients with MTC. DCs were established from mononuclear leukocytes by culturing in the presence of Flt-3 ligand，GM-CSF, I卜4, and TNF- a for 14 days. At day 14，the differentiated DCs was analyzed morphologically. The immunophenotypic features of DCs such as CDla，CD83, and CD86 were analyzed by Immunofluorelescence microscopy. At day 15, DCs were incubated with thyroid cancer tissues and normal thyroid tissues for 7 additional days，respectively.


Results : DCs were generated from the peripheral blood mononuclear cells. The generated cells showed the typical morphology of DCs. Activated cytotoxic T lymphocytes (CTLs) were observed. DCs attached to the thyroid cancer tissues and the CTLs were attached to the MTC tissues on scanning electron microscope.


Conclusion : We could differentiate DCs from the peripheral blood mononuclear cells，And the DCs activate the CTLs which able to attack the MTC tissues. These results suggest that DCs can be used as adjuvants for immunotherapy of MTC. And this study represent the basis for the develop of new therapeutic strategies not only in MTC but also in the other malignancies.

영어초록

Background : Recent studies suggest that immunization with autologous dendritic cells(DCs) result in protective immunity and rejection of established tumors in various human malignancies, It has been reported that a dense infiltration of dendritic cells correlates with a favorable prognosis in several types of cancer. The purpose of this study is to determine whether DCs are generated from peripheral blood monocytes by using cytokines such as Fit-3 ligand， granulocyte macrophage-colony stimulating factor (GM-CSF), IL-4, and TNF- a，and whether cytotoxic T cells activated against the medullary thyroid carcinoma(MTC) tissues by the DCs.




Methods : Peripheral blood was obtained from 2 patients with MTC. DCs were established from mononuclear leukocytes by culturing in the presence of Flt-3 ligand，GM-CSF, I卜4, and TNF- a for 14 days. At day 14，the differentiated DCs was analyzed morphologically. The immunophenotypic features of DCs such as CDla，CD83, and CD86 were analyzed by Immunofluorelescence microscopy. At day 15, DCs were incubated with thyroid cancer tissues and normal thyroid tissues for 7 additional days，respectively.




Results : DCs were generated from the peripheral blood mononuclear cells. The generated cells showed the typical morphology of DCs. Activated cytotoxic T lymphocytes (CTLs) were observed. DCs attached to the thyroid cancer tissues and the CTLs were attached to the MTC tissues on scanning electron microscope.




Conclusion : We could differentiate DCs from the peripheral blood mononuclear cells，And the DCs activate the CTLs which able to attack the MTC tissues. These results suggest that DCs can be used as adjuvants for immunotherapy of MTC. And this study represent the basis for the develop of new therapeutic strategies not only in MTC but also in the other malignancies.

참고자료

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