돼지에서 난자의 체외발육 배양액 내 첨가된 거대분자물질이작은 난포 유래 미성숙 난자의 성장, 성숙 및 배 발육에 미치는 영향 (Various macromolecules in in vitro growth medium influence growth, maturation, and parthenogenetic development of pig oocytes derived from small antral follicles)

서지정보

· 발행기관 : 대한수의학회
· 수록지 정보 : 대한수의학회지 / 59권 / 2호 / 81 ~ 88페이지
· 저자명 : 이한나, 이용진, 이주형, 이근식, 이승태, 이은송

초록

This study was performed to examine the effects of various macromolecules in in vitro growth (IVG) media on the growth,maturation, and parthenogenesis (PA) of pig oocytes derived from small antral follicles (SAF). Immature oocytes were cultured for twodays in IVG medium supplemented with 10% (v/v) fetal bovine serum (FBS), 10% (v/v) pig follicular fluid (PFF), 0.4% (w/v) bovineserum albumin (BSA), or 0.1% (w/v) polyvinyl alcohol (PVA) and then maintained for 44 h for maturation. After IVG, the meandiameters of the SAF treated with FBS, PVA, and no IVG-MAF (113.0–114.8 μm) were significantly larger than that of no IVG-SAF(111.8 μm). The proportion of metaphase II oocytes was higher in PFF (73.6%) than in BSA (43.5%) and PVA (53.7%) but similar tothat in the FBS treatment (61.5%). FBS and PFF increased cumulus expansion significantly compared to PVA and BSA while the intraoocyteglutathione content was not influenced by the macromolecules. Blastocyst formation of PA oocytes treated with FBS (51.8%),PFF (50.4%), and PVA (45.2%) was significantly higher than that of the BSA-treated oocytes (20.6%). These results show that the PFFand FBS treatments during IVG improved the growth, maturation, and embryonic development of SAF.

영어초록

This study was performed to examine the effects of various macromolecules in in vitro growth (IVG) media on the growth,maturation, and parthenogenesis (PA) of pig oocytes derived from small antral follicles (SAF). Immature oocytes were cultured for twodays in IVG medium supplemented with 10% (v/v) fetal bovine serum (FBS), 10% (v/v) pig follicular fluid (PFF), 0.4% (w/v) bovineserum albumin (BSA), or 0.1% (w/v) polyvinyl alcohol (PVA) and then maintained for 44 h for maturation. After IVG, the meandiameters of the SAF treated with FBS, PVA, and no IVG-MAF (113.0–114.8 μm) were significantly larger than that of no IVG-SAF(111.8 μm). The proportion of metaphase II oocytes was higher in PFF (73.6%) than in BSA (43.5%) and PVA (53.7%) but similar tothat in the FBS treatment (61.5%). FBS and PFF increased cumulus expansion significantly compared to PVA and BSA while the intraoocyteglutathione content was not influenced by the macromolecules. Blastocyst formation of PA oocytes treated with FBS (51.8%),PFF (50.4%), and PVA (45.2%) was significantly higher than that of the BSA-treated oocytes (20.6%). These results show that the PFFand FBS treatments during IVG improved the growth, maturation, and embryonic development of SAF.

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