중부지방 적합 자운영 (Astragalus sinicus L.) 형질 특성 및 유전적 연관성 분석에 관한 연구 (Study for Morphological and Genetic Characteristics of Chinese Milk Vetch (Astragalus sinicus L.) to Select Suitable Line in Central Area of Korea)

서지정보

· 발행기관 : 한국환경생물학회
· 수록지 정보 : 환경생물 / 34권 / 3호 / 169 ~ 176페이지
· 저자명 : 홍선희, 김재윤

초록

본 연구는 중북부지방 적응성 자운영 선발 및 수집 계통간 형태적, 유전적 근연관계를 분석한 것으로서 총 수집된16개 자운영 계통 중 파주종은 엽각이 적고, 지하부의 발육이 좋아 형태적 형질이 월동에 적합한 초형이었다. 또한 구례 수집종은 개화기가 타 수집종에 비해 일주일 가량 빨랐다. 유전적 근연관계를 검정하기 위해 8개 primer 조합으로AFLP를 실시한 결과 총 579개의 밴드를 얻었으며, 이 중polymorphism을 갖는 밴드는 61.54%인 336개 밴드였다.
AFLP 데이터를 이용해 군집분석을 실시한 결과 계통 간 유사도 지수는 0.826~0.939 사이의 값을 가지며, 구례와 파주수집종은 타 수집종과 유전적 유사도에서 비교적 큰 차이를보였다.

영어초록

Although chemical fertilizers have a quick effect and broad applicability to agricultural fields, they have caused many problems like increasing soil acidity or decreasing soil organic matters. Environmental-friendly agriculture has been attempted in various ways such as organic agriculture, natural farming, low input and sustainable agriculture. The common interest of all environmental-friendly systems is to decrease burden to agricultural environment by low input of agricultural labor and materials. This study was conducted to estimate overwintering capacity and genetic distance among Chinese Milk Vetch (Astragalus sinicus, CMV) collections based on morphological characteristics and AFLP (Amplified fragment length polymorphism) analysis. Furthermore, the effect of CMV as green manure was observed in mix-cultured paddy fields with rice, sesame and sweet-potato. An another objective of this study was also to compare the pattern of weed occurrence in paddy fields with or without CMV and different rice transplanting times. The CMV collected from Paju district in central region of Korea was successively occurring through self-reseeding without artificial management. However, there was no noticeable difference in growth habit between Paju native CMV and introduced CMV from China which is currently used in farm fields. On the basis of multi-dimensional scaling and tree analyses, there are no significant difference of agricultural growth characteristics among Paju and chinese collections only excepting leaf angle and root length. The flowering time of Gurye collection was fast for 1 week as compared to other collections. AFLP that was commonly used for plant classfication, was applied to exam the genetic variation of CMV collections. Total 579 PCR products and 336 polymorphic fragments were generated using 8 primer pairs.

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