배혈에 따른 저빈도 전침자극이 류마토이드 관절염 유발 흰쥐의 PAG와 해마부위에서 NOS 발현에 미치는 영향 (Effect of Low Frequency Electroacupuncuture According to Acupoint Combination on NOS Positive Neurons in the PAG and Hippocampus of Rat with Adjuvant Induced Rheumatoid Arthritis)

서지정보

· 발행기관 : 대한침구의학회
· 수록지 정보 : Journal of Acupuncture Research / 25권 / 4호 / 1 ~ 11페이지
· 저자명 : 황희상, 김이화, 노정두, 이은용

초록

Objectives : This study was to investigate the effect of low frequency electroacupuncture according to acupoints combination on NOS positive neurons in the PAG and Hippocampus of Rat with adjuvant induced Rheumatoid arthritis.
Methods : Arthritis was induced by intradermal injection of FCA into base of tail. Experimental group were divided into 6 group ; Normal, Control, ST36, SP9, ST36+SP9, and NA.
Normal group was injected with normal saline and not treated electroacupuncture. Control group was injected with FCA and not treated electroacupuncture. ST36 group was injected with FCA and treated by 2 Hz electroacupuncture at ST36. SP9 group was injected with FCA and treated by 2 Hz electroacupuncture at SP9. ST36+SP9 group was injected with FCA and treated by 2 Hz electroacupuncture at ST36+SP9. NA group was injected with FCA and treated by 2 Hz electroacupuncture at non acupoint. Each groups were evaluated by the number of NOS-positive neurons in PAG and hippocampus by using an image analyzer and a microscope.
Results :
1. The number of NOS-positive neurons of the NA group were significantly decreased in the CA2-3.
2. The number of NOS-positive neurons of the ST36 group were significantly decreased in the PAG and CA2-3.
3. The number of NOS-positive neurons of the SP9 group were significantly decreased in the PAG and DG.
4. The number of NOS-positive neurons of the ST36+SP9 group were significantly decreased in the PAG, CA1 and DG.
Conclusions : It is expected that low frequency electroacupuncture can be used a treatment of rheumatoid arthritic pain and that treatment of electroacupuncture at combined acupoints is more efficient than treatment of low frequency electroacupuncture at one acupoint.

영어초록

Objectives : This study was to investigate the effect of low frequency electroacupuncture according to acupoints combination on NOS positive neurons in the PAG and Hippocampus of Rat with adjuvant induced Rheumatoid arthritis.
Methods : Arthritis was induced by intradermal injection of FCA into base of tail. Experimental group were divided into 6 group ; Normal, Control, ST36, SP9, ST36+SP9, and NA.
Normal group was injected with normal saline and not treated electroacupuncture. Control group was injected with FCA and not treated electroacupuncture. ST36 group was injected with FCA and treated by 2 Hz electroacupuncture at ST36. SP9 group was injected with FCA and treated by 2 Hz electroacupuncture at SP9. ST36+SP9 group was injected with FCA and treated by 2 Hz electroacupuncture at ST36+SP9. NA group was injected with FCA and treated by 2 Hz electroacupuncture at non acupoint. Each groups were evaluated by the number of NOS-positive neurons in PAG and hippocampus by using an image analyzer and a microscope.
Results :
1. The number of NOS-positive neurons of the NA group were significantly decreased in the CA2-3.
2. The number of NOS-positive neurons of the ST36 group were significantly decreased in the PAG and CA2-3.
3. The number of NOS-positive neurons of the SP9 group were significantly decreased in the PAG and DG.
4. The number of NOS-positive neurons of the ST36+SP9 group were significantly decreased in the PAG, CA1 and DG.
Conclusions : It is expected that low frequency electroacupuncture can be used a treatment of rheumatoid arthritic pain and that treatment of electroacupuncture at combined acupoints is more efficient than treatment of low frequency electroacupuncture at one acupoint.

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