위령선약침이 Collagen, Adjuvant, LPS 및 PLA2 유발 류머티스성 관절염에 미치는 영향 (The Effect of Clematidis Radix Herbal-acupuncture Solution, on Collagen, Adjuvant, Lipopolysaccharide and Phospholipase A2 Induced Rheumatoid Arthritis in Mice)

서지정보

· 발행기관 : 대한침구의학회
· 수록지 정보 : Journal of Acupuncture Research / 29권 / 1호 / 127 ~ 137페이지
· 저자명 : 이진석, 김경호, 이승덕, 김갑성

초록

Objectives : The purpose of this study is to investigate the effect of Clematidis radix herbal-acupuncture solution, on collagen, adjuvant, lipopolysaccharide and phospholipase A2 induced rheumatoid arthritis in mice.
Methods : Arthritis index was measured for mouse that was injected subcutaneously in solution mixed chicken type Ⅱ collagen with Freund’s complete adjuvant. We injected Freund’s complete adjuvant into right posterior part of the sole of a ICR mouse foot, which was measured by plethysmometer. The solution mixed CRHS with Tris-HCI, CaCl2, substrate, enzyme was done a chemical action for thirty minutes, and then inhibitory activity of PLA2 enzyme was expressed with inhibition percentage by utilizing isolated arachidonic acid. COX-2 was induced by adding LPS to RAW 264.7 cell, and COX-2 activity was measured by western blot analysis and PGE2 Biotrak kit.
Results : CRHS also inhibited Freund’s complete adjuvant induced chronic rheumatoid arthritis in mice. CRHS showed significant inhibition of type Ⅰ and type Ⅱ PLA2 activities in a dose dependent manner. Furthermore, PGE2 production was decreased with CRHS and lipopolysaccharide-induced COX-2 protein expression was significantly inhibited by CRHS.
Conclusions : These results suggest that CRHS has an therapeutic effect on drug induced-rheumatoic arthritis by inhibiting PLA2 and COX-2 activities.

영어초록

Objectives : The purpose of this study is to investigate the effect of Clematidis radix herbal-acupuncture solution, on collagen, adjuvant, lipopolysaccharide and phospholipase A2 induced rheumatoid arthritis in mice.
Methods : Arthritis index was measured for mouse that was injected subcutaneously in solution mixed chicken type Ⅱ collagen with Freund’s complete adjuvant. We injected Freund’s complete adjuvant into right posterior part of the sole of a ICR mouse foot, which was measured by plethysmometer. The solution mixed CRHS with Tris-HCI, CaCl2, substrate, enzyme was done a chemical action for thirty minutes, and then inhibitory activity of PLA2 enzyme was expressed with inhibition percentage by utilizing isolated arachidonic acid. COX-2 was induced by adding LPS to RAW 264.7 cell, and COX-2 activity was measured by western blot analysis and PGE2 Biotrak kit.
Results : CRHS also inhibited Freund’s complete adjuvant induced chronic rheumatoid arthritis in mice. CRHS showed significant inhibition of type Ⅰ and type Ⅱ PLA2 activities in a dose dependent manner. Furthermore, PGE2 production was decreased with CRHS and lipopolysaccharide-induced COX-2 protein expression was significantly inhibited by CRHS.
Conclusions : These results suggest that CRHS has an therapeutic effect on drug induced-rheumatoic arthritis by inhibiting PLA2 and COX-2 activities.

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