Spargue-Dawley 랫드를 이용한 구미강활탕의 급성독성 연구 (Acute Toxicity Study on Gumiganghwal-tang (Jiuweiqianghuo-tang) in Sprague-Dawley Rats)

서지정보

· 발행기관 : 대한한의학방제학회
· 수록지 정보 : 대한한의학 방제학회지 / 18권 / 1호 / 79 ~ 85페이지
· 저자명 : 신인식, 김정훈, 하혜경, 서창섭, 이미영, 이호영, 이준경, 이남헌, 이진아, 이설림, 허정임, 신현규

초록

Objectives : This study was conducted to evaluate the acute toxicity and safety of Gumiganghwal -tang (Jiuweiqianghou-tang) in Sprague-Dawley rats though the current regulatory guideline.
Methods : The preliminary study showed that the single oral administration of Gumiganghwal-tang (Jiuweiqianghou-tang) did not induce any toxic effect at a dose level of 2000 mg/kg. Based on the results, 2000 mg/kg was selected as the limited dose. In this study, 10 rats of each sex were randomly assigned to two groups of 5 rats each and were administrated singly by gavage at dose levels of 0 and 2000 mg/kg. Mortalities, clinical signs, and body weight changes were monitored for the 15-day period following administration. At the end of observation period, all animals were sacrificed and complete gross postmortem examinations were performed. `Results : Throughout the study period, no treatment-related deaths were observed. There were no adverse effects on clinical signs, body weight, and gross findings at all treatment groups.
Conclusions : These results showed that the single oral adminstration of Gumiganghwal-tang (Jiuweiqianghou-tang) did not cause any toxic effect at the dose levels of 2000 mg/kg in rats. In conclusion, the LD50 of Gumiganghwal-tang (Jiuweiqianghou-tang) was considered to be over 2000 mg/kg body for both sexes.

영어초록

Objectives : This study was conducted to evaluate the acute toxicity and safety of Gumiganghwal -tang (Jiuweiqianghou-tang) in Sprague-Dawley rats though the current regulatory guideline.
Methods : The preliminary study showed that the single oral administration of Gumiganghwal-tang (Jiuweiqianghou-tang) did not induce any toxic effect at a dose level of 2000 mg/kg. Based on the results, 2000 mg/kg was selected as the limited dose. In this study, 10 rats of each sex were randomly assigned to two groups of 5 rats each and were administrated singly by gavage at dose levels of 0 and 2000 mg/kg. Mortalities, clinical signs, and body weight changes were monitored for the 15-day period following administration. At the end of observation period, all animals were sacrificed and complete gross postmortem examinations were performed. `Results : Throughout the study period, no treatment-related deaths were observed. There were no adverse effects on clinical signs, body weight, and gross findings at all treatment groups.
Conclusions : These results showed that the single oral adminstration of Gumiganghwal-tang (Jiuweiqianghou-tang) did not cause any toxic effect at the dose levels of 2000 mg/kg in rats. In conclusion, the LD50 of Gumiganghwal-tang (Jiuweiqianghou-tang) was considered to be over 2000 mg/kg body for both sexes.

참고자료

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