당귀로부터 정제한 Decursin의 인간 급성 단핵구성백혈병 세포(THP-1 cells)의 세포 독성 및 Apoptosis에 미치는 영향 (Decursin from Angelica gigas Nakai Promotes Cytotoxicity and Induces Apoptosis in THP-1 cells, a Human Acute Monocytic Leukemia)

서지정보

· 발행기관 : 한국생약학회
· 수록지 정보 : 생약학회지 / 47권 / 3호 / 197 ~ 203페이지
· 저자명 : 김남석, 정승일, 김종석, 오미진, 오찬호

초록

Decursin is a major component of the root of Angelica gigas(Umbelliferae), which has been traditionally used in Korea as a tonic and to treat anemia, hemiplegia, and women’s diseases. The objective of this study is to identify the anti-cancer mechanism induced by decursin on apoptosis of human leukemia and lymphoma cells. Cytotoxicity of decursin on U937, HL-60, MOLT-4, THP-1 cells showed the significant effects. First of all, IC50 of decursin on four cell lines was 27.1, 32.4, 17.4, 15.1 mM, respectively. So IC50 in THP-1 cells was the smallest among 4 cell lines treated with decursin(15.1 mM). In order to understand the apoptosis-mechanism by decursin, we examined the gene expression of bcl-2(anti-apoptotic), bax(pro-apoptotic)and p53(tumor suppressor)after treating the THP-1 cells with decursin(10, 50 and 100 mM). It was found bcl-2 gene was decreased dose dependently, the expression level of bax gene of THP-1 cells treated with 100 mM of decursin was about 3 times higher than those of control, and p53 gene was increased In the same concentration(100 mM), p53 gene was increased dose dependent manner. In protein express, bcl-2 and p53 protein showed a tendency to decrease. bax was increased about 4 fold. Therefore decursin is a useful chemotherapeutic agent against leukemia.

영어초록

Decursin is a major component of the root of Angelica gigas(Umbelliferae), which has been traditionally used in Korea as a tonic and to treat anemia, hemiplegia, and women’s diseases. The objective of this study is to identify the anti-cancer mechanism induced by decursin on apoptosis of human leukemia and lymphoma cells. Cytotoxicity of decursin on U937, HL-60, MOLT-4, THP-1 cells showed the significant effects. First of all, IC50 of decursin on four cell lines was 27.1, 32.4, 17.4, 15.1 mM, respectively. So IC50 in THP-1 cells was the smallest among 4 cell lines treated with decursin(15.1 mM). In order to understand the apoptosis-mechanism by decursin, we examined the gene expression of bcl-2(anti-apoptotic), bax(pro-apoptotic)and p53(tumor suppressor)after treating the THP-1 cells with decursin(10, 50 and 100 mM). It was found bcl-2 gene was decreased dose dependently, the expression level of bax gene of THP-1 cells treated with 100 mM of decursin was about 3 times higher than those of control, and p53 gene was increased In the same concentration(100 mM), p53 gene was increased dose dependent manner. In protein express, bcl-2 and p53 protein showed a tendency to decrease. bax was increased about 4 fold. Therefore decursin is a useful chemotherapeutic agent against leukemia.

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