올로파타딘이 토끼 결막세포에 미치는 독성 비교 (Comparison of the Toxicity of Olopatadine Anti-allergic Ophthalmic Agents on Rabbit Conjunctival Cells)

서지정보

· 발행기관 : 대한안과학회
· 수록지 정보 : 대한안과학회지 / 60권 / 12호 / 1176 ~ 1184페이지
· 저자명 : 정영환, 장수경, 이종수

초록

목적: 임상에 사용되는 항알레르기 약제인 올로파타딘의 농도 및 노출 시간에 따라 토끼 결막세포에 독성을 얼마나 미치는지에 대해 비교하고자 하였다.
대상과 방법: 배양된 토끼의 결막세포에 Patanol® (0.1% olopatadine hydrochloride; Alcon, Fort Worth, TX, USA), Pataday® (0.2% olopatadine hydrochloride; Alcon), Pazeo® (0.7% olopatadine hydrochloride; Alcon)를 사용하여 각각 5%, 10%, 15% 농도별로 30분, 1시간, 2시간, 3시간, 6시간 접촉시킨 후 비교하였다. Methylthiazoltetrazolium, lactate dehydrogenase를 측정하였으며, 약제의 구성성분과 더불어 광학 및 전자현미경을 사용하여 세포의 형태학적인 변화를 비교 관찰하였다.
결과: 세 약제 모두 15% 농도에서 현저한 세포증식 억제를 나타냈고, Pataday®, Pazeo®는 10% 농도에서도 노출 6시간이 지나면 세포의 증식을 현저히 억제하였다. 젖산탈수소효소 수치는 약제 노출 3시간째부터 증가하였는데, 15% pazeo®는 2시간째부터 젖산탈수소효소 수치가 증가하였다. Olopatadine hydrochloride 농도가 높은 Pazeo®, Pataday®, Patanol® 순으로 결막세포의 형태적 변성이 나타났다.
결론: 올라파타딘 계열의 약제 중 농도가 높은 Pazeo®, Pataday®, Patanol® 순으로 결막세포의 손상이 초래되었지만, 심각한 손상인 세포질막이나 핵막의 손상은 없었다.

영어초록

Purpose: To compare the in vitro toxicity of commercial olopatadine anti-allergic ophthalmic agents on cultured rabbit’s conjunctival cells according to concentrations and exposure times.
Methods: Rabbit conjunctival cells were exposed to anti-allergic olopatadine ophthalmic agents, (Patanol® [0.1% olopatadine hydrochloride; Alcon, Fort Worth, TX, USA], Pataday® [0.2% olopatadine hydrochloride; Alcon], and Pazeo® [0.7% olopatadine hydrochloride; Alcon]) at concentrations of 5%, 10%, and 15% for periods of 30 minutes, 1, 2, 3, and 6 hours, respectively. Cell proliferation and injury assays were performed using the methylthiazoltetrazolium and lactate dehydrogenase (LDH) leakage assays. We checked the composition of the three anti-allergic agents, and performed light and transmission electron microscopy to compare the morphological changes in cells.
Results: The conjunctival cell proliferation was inhibited after 1 hour exposure to each olopatadine ophthalmic agent, with significant cell proliferation inhibited using 15% of each drug. The proliferation of conjunctival cells was inhibited during 6 hours of drug exposure at all concentrations of Pataday® and Pazeo®. The titer of LDH increased from 3 hours after drug exposure, but 15% Pazeo® significantly increased the LDH titer at 2 hours after drug exposure. As the concentration of the drug increased, the LDH titer also significantly increased. The cellular morphological changes of conjunctival cells were in the increasing order of Pazeo®, Pataday®, and Patanol® with a high concentration of olopatadine hydrochloride.
Conclusions: Among the anti-allergic olopatidine ophthalmic agents, higher olopatadine concentrations in the increasing order of Pazeo®, Pataday®, and Patanol® resulted in cytoplasmic damage of conjunctival cells, but there was no severe damage to the cytoplasmic or the nuclear membranes.

참고자료

· 없음