朱雀丸이 일시적 국소 뇌허혈 유발 白鼠에 대한 인지 및 운동기능 회복에 미치는 효과 (The Recovery Effects of Joojakwhan - A Traditional Korean Medical Poly-herbal Drug for the Cognitions and Motor Functions in Mild Stroke Rat Model by Using Transient Middle Cerebral Artery Occlusion)

서지정보

· 발행기관 : 대한한방신경정신과학회
· 수록지 정보 : 동의신경정신과학회지 / 24권 / 4호 / 419 ~ 434페이지
· 저자명 : 김보은, 강석봉, 정대규

초록

Objectives: The object of this study is to observe the cognition and motor function recovery effects of Joojakwhan (JJW), a traditional Korean poly-herbal formula for treating various neuropsychiatric dis-eases such as dementia, for the mildly stroke rats , with 60 minutes of reperfusion transient middle cer-ebral artery occlusion (tMCAO).Methods: In the present study, 125, 250 and 500 mg/kg of JJW were orally administered, once per day for 10 continuous days 2 hours after the tMCAO. The body weight changes, infarct sizes under 2% 2, 3, 5-triphenyl tetrazolium chloride (TTC) stain, sensorimotor functions and cognitive motor behavior tests were serially monitored with cerebral caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP)-immunoreactivities and histopathological changes. The effects of tMCAO on sensorimotor functions were evaluated by using of limb placing and body-swing tests, and the cognitive motor be-haviors were also observed with water maze tests.Results: From the results of tMCAO, with marked decreases of body weights, disorders of sensorimotor functions increases the limb placing test scores, and decrease the numbers and percentages of body swings to the ipsilateral sides. The cognitive motor behaviors increases the distances and time to reach the escape platform which included the inhibitions of the decreases with repeated trials that were ob-served with focal cerebral cortex infarct volumes. In addition, the marked increases of the atrophy, numbers of degeneration, caspase-3- and PARP-immunoreactive cells around peri-infarct ipsilateral cerebral cortex were also observed in tMCAO controls when compared with the sham control rats, respectively. Conclusions: The results obtained from this study suggest that oral administrations of JJW indicate ob-vious cognitions and motor function recoveries of the rats with tMCAO, mild strokes, which are medi-ated by neuro-protective effects through known antioxidant effects of components.

영어초록

Objectives: The object of this study is to observe the cognition and motor function recovery effects of Joojakwhan (JJW), a traditional Korean poly-herbal formula for treating various neuropsychiatric dis-eases such as dementia, for the mildly stroke rats , with 60 minutes of reperfusion transient middle cer-ebral artery occlusion (tMCAO).Methods: In the present study, 125, 250 and 500 mg/kg of JJW were orally administered, once per day for 10 continuous days 2 hours after the tMCAO. The body weight changes, infarct sizes under 2% 2, 3, 5-triphenyl tetrazolium chloride (TTC) stain, sensorimotor functions and cognitive motor behavior tests were serially monitored with cerebral caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP)-immunoreactivities and histopathological changes. The effects of tMCAO on sensorimotor functions were evaluated by using of limb placing and body-swing tests, and the cognitive motor be-haviors were also observed with water maze tests.Results: From the results of tMCAO, with marked decreases of body weights, disorders of sensorimotor functions increases the limb placing test scores, and decrease the numbers and percentages of body swings to the ipsilateral sides. The cognitive motor behaviors increases the distances and time to reach the escape platform which included the inhibitions of the decreases with repeated trials that were ob-served with focal cerebral cortex infarct volumes. In addition, the marked increases of the atrophy, numbers of degeneration, caspase-3- and PARP-immunoreactive cells around peri-infarct ipsilateral cerebral cortex were also observed in tMCAO controls when compared with the sham control rats, respectively. Conclusions: The results obtained from this study suggest that oral administrations of JJW indicate ob-vious cognitions and motor function recoveries of the rats with tMCAO, mild strokes, which are medi-ated by neuro-protective effects through known antioxidant effects of components.

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