지방조직 유래 줄기세포의 조골세포로의 분화에 대한 실험적 연구 (A study on the osteogenic differentiation of adipose-derived adult stem cell)

서지정보

· 발행기관 : 대한악안면성형재건외과학회
· 수록지 정보 : Maxillofacial Plastic Reconstructive Surgery / 30권 / 2호 / 133 ~ 141페이지
· 저자명 : 이의석, 장현석, 권종진, 임재석

초록

Stem cells have self-renewal capacity, long-term viability, and multilineage potential. Adult bone
marrow contains mesenchymal stem cells. Bone marrow-derived mesenchymal stem cells (BMSCs)
are progenitors of skeletal tissue components and can differentiate into adipocytes, chondrocytes,
osteoblasts, and myoblasts in vitro and undergo differentiation in vivo.
However, the clinical use of BMSCs has presented problems, including pain, morbidity, and low
cell number upon harvest. Recent studies have identified a putative stem cell population within the
adipose tissue. Human adipose tissue contains pluripotent stem cells simillar to bone marrowderived
stem cells that can differentiate toward the osteogenic, adipogenic, myogenic, and chondrogenic
lineages.
Human adipose tissue-derived stem cells (ATSCs) could be proposed as an alternative source of
adult bone marrow stem cells, and could be obtained in large quantities, under local anesthesia,
with minimal discomfort. Human adipose tissue obtained by liposuction was processed to obtain
ATSCs.
In this study, we compared the osteogenic differentiation of ATSCs in a specific osteogenic induction
medium with that in a non-osteogenic medium. ATSCs were incubated in an osteogenic medium
for 28 days to induce osteogenesis respectively. Osteogenic differentiation was assessed by von Kossa
and alkaline phosphatase staining. Expression of osteocyte specific bone sialoprotein, osteocalcin,
collagen type I and alkaline phosphatase, bone morphogenic protein 2, bone morphogenic protein 6
was confirmed by RT-PCR.
ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline
phosphatase staining. Expression of osteocyte specific genes was also detected. Since this cell population
can be easily identified through fluorescence microscopy, it may be an ideal source of ATSCs
for further experiments on stem cell biology and tissue engineering. The present results show that
ADSCs have an ability to differentiate into osteoblasts. In the present study, we extend this
approach to characterize adipose tissue-derived stem cells.

영어초록

Stem cells have self-renewal capacity, long-term viability, and multilineage potential. Adult bone
marrow contains mesenchymal stem cells. Bone marrow-derived mesenchymal stem cells (BMSCs)
are progenitors of skeletal tissue components and can differentiate into adipocytes, chondrocytes,
osteoblasts, and myoblasts in vitro and undergo differentiation in vivo.
However, the clinical use of BMSCs has presented problems, including pain, morbidity, and low
cell number upon harvest. Recent studies have identified a putative stem cell population within the
adipose tissue. Human adipose tissue contains pluripotent stem cells simillar to bone marrowderived
stem cells that can differentiate toward the osteogenic, adipogenic, myogenic, and chondrogenic
lineages.
Human adipose tissue-derived stem cells (ATSCs) could be proposed as an alternative source of
adult bone marrow stem cells, and could be obtained in large quantities, under local anesthesia,
with minimal discomfort. Human adipose tissue obtained by liposuction was processed to obtain
ATSCs.
In this study, we compared the osteogenic differentiation of ATSCs in a specific osteogenic induction
medium with that in a non-osteogenic medium. ATSCs were incubated in an osteogenic medium
for 28 days to induce osteogenesis respectively. Osteogenic differentiation was assessed by von Kossa
and alkaline phosphatase staining. Expression of osteocyte specific bone sialoprotein, osteocalcin,
collagen type I and alkaline phosphatase, bone morphogenic protein 2, bone morphogenic protein 6
was confirmed by RT-PCR.
ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline
phosphatase staining. Expression of osteocyte specific genes was also detected. Since this cell population
can be easily identified through fluorescence microscopy, it may be an ideal source of ATSCs
for further experiments on stem cell biology and tissue engineering. The present results show that
ADSCs have an ability to differentiate into osteoblasts. In the present study, we extend this
approach to characterize adipose tissue-derived stem cells.

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