실크 피브로인 지지체와 Substance P를 이용한 골 이식재 (Silk Fibroin and Substance P Combination Graft for the Reconstruction of a Bone Defect)

서지정보

· 발행기관 : 대한악안면성형재건외과학회
· 수록지 정보 : Maxillofacial Plastic Reconstructive Surgery / 33권 / 4호 / 293 ~ 300페이지
· 저자명 : 박기유, 최교희, 박영주, 송지영, 김성곤, 조유영, 권해용, 강석우

초록

Purpose: Substance P is a well known neurotransmitter and has been known to mediate pain. Recently, it has been unveiled that substance P is involved in the recruitment of mesenchymal stem cells to wound sites. The purpose of this study was to exam bone formation when a combination of substance P and silk fibroin was used in a bone defect model.
Methods: Twenty rabbits were used and 40 calvarial defects were formed. They were divided as 4 groups (unfilled control,silk only, silk＋10μg/ml substance P; Sub10, and silk＋100μg/ml substance P; Sub100). All animals were humanely sacrificed 4 or 8 weeks after grafting. The specimens were analyzed by micro-computerized tomography and histological analysis.
Results: When compared to the unfilled control to silk only group, there was significant difference in bone mineral density (BMD) and the attenuation coefficient (AC) at 4 weeks (P=0.037 and 0.038, respectively). When compared Sub10 group to Sub100 group, there was significant difference in BMD and AC at 8 weeks (P=0.004 for all). Residual graft amounts were 52.1±15.8%, 15.2±9.2% and 9.0±3.3% for silk only, Sub10, and Sub100 groups, respectively. When comparing the residual graft amount of silk only to sub10 or sub100, the differences were statistically significant (P＜0.001).
Conclusion: The silk fibroin scaffold showed higher BMD and AC than the unfilled control. The combination graft with substance P and silk fibroin scaffold showed a faster graft degradation than with a silk fibroin scaffold only.

영어초록

Purpose: Substance P is a well known neurotransmitter and has been known to mediate pain. Recently, it has been unveiled that substance P is involved in the recruitment of mesenchymal stem cells to wound sites. The purpose of this study was to exam bone formation when a combination of substance P and silk fibroin was used in a bone defect model.
Methods: Twenty rabbits were used and 40 calvarial defects were formed. They were divided as 4 groups (unfilled control,silk only, silk＋10μg/ml substance P; Sub10, and silk＋100μg/ml substance P; Sub100). All animals were humanely sacrificed 4 or 8 weeks after grafting. The specimens were analyzed by micro-computerized tomography and histological analysis.
Results: When compared to the unfilled control to silk only group, there was significant difference in bone mineral density (BMD) and the attenuation coefficient (AC) at 4 weeks (P=0.037 and 0.038, respectively). When compared Sub10 group to Sub100 group, there was significant difference in BMD and AC at 8 weeks (P=0.004 for all). Residual graft amounts were 52.1±15.8%, 15.2±9.2% and 9.0±3.3% for silk only, Sub10, and Sub100 groups, respectively. When comparing the residual graft amount of silk only to sub10 or sub100, the differences were statistically significant (P＜0.001).
Conclusion: The silk fibroin scaffold showed higher BMD and AC than the unfilled control. The combination graft with substance P and silk fibroin scaffold showed a faster graft degradation than with a silk fibroin scaffold only.

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