미니돼지에서 자가 피부유래 전구세포와 탈회골 및 피브린 스케폴드를 이용한 하악골 골결손부의 골재생에 대한 연구 (MANDIBULAR BONE REGENERATION USING AUTOGENOUS SKIN-DERIVED PRECURSOR CELLS WITH A MIXED DEMINERALIZED BONE AND FIBRIN GLUE SCAFFOLD IN MINIATURE PIGS)

서지정보

· 발행기관 : 대한악안면성형재건외과학회
· 수록지 정보 : Maxillofacial Plastic Reconstructive Surgery / 31권 / 3호 / 198 ~ 206페이지
· 저자명 : 변준호, 김욱규, 김종렬, 박봉욱, 최문정, 최영진, 심경목

초록

Purpose: The aims of this study were to assess the in vitro co-culturing pattern of isolated skin-derived
precursor cells (SKPs) with a mixed demineralized bone (DMB) and fibrin glue scaffold and to evaluate in
vivo osteogenesis after transplantation of autogenous SKPs with a these mixed scaffold in the animal’s
mandibular defects.
Materials and Methods: We isolated SKPs from the ears of adult 4 miniature pigs. The isolated SKPs
were co-cultured with a mixed DMB and fibrin glue scaffold in a non-osteogenic medium for 1, 2, and 4
weeks. Histological characteristics of in vitro co-cultured cells and scaffold were evaluated. 1x107 cells/100
μl of autogenous porcine SKPs were grafted into the mandibular defects with a DMB and fibrin glue scaffold.
In the control sites, only a scaffold was grafted, without SKPs. After two animals each were euthanized
at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric
and osteocalcin immunohistochemical studies.
Results: Homogeneously shaped skin-derived cells were isolated from porcine ear skin after 3 or 4
weeks of primary culture. In vitro osteogenic differentiation of SKPs was observed after co-culturing with a
DMB and fibrin glue scaffold in a non-osteogenic medium. Von Kossa-positive bone minerals were also
noted in the co-cultured medium at 4 weeks. As the culture time progressed, the number of observable
cells increased. Trabecular new bone formation and osteocalcin expression were more pronounced in the
SKP-grafted group compared to the control group.
Conclusion: These findings suggest that autogenous SKP grafting with a DMB and fibrin glue scaffold
can serve as a useful alternative to bone grafting technique.

영어초록

Purpose: The aims of this study were to assess the in vitro co-culturing pattern of isolated skin-derived
precursor cells (SKPs) with a mixed demineralized bone (DMB) and fibrin glue scaffold and to evaluate in
vivo osteogenesis after transplantation of autogenous SKPs with a these mixed scaffold in the animal’s
mandibular defects.
Materials and Methods: We isolated SKPs from the ears of adult 4 miniature pigs. The isolated SKPs
were co-cultured with a mixed DMB and fibrin glue scaffold in a non-osteogenic medium for 1, 2, and 4
weeks. Histological characteristics of in vitro co-cultured cells and scaffold were evaluated. 1x107 cells/100
μl of autogenous porcine SKPs were grafted into the mandibular defects with a DMB and fibrin glue scaffold.
In the control sites, only a scaffold was grafted, without SKPs. After two animals each were euthanized
at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric
and osteocalcin immunohistochemical studies.
Results: Homogeneously shaped skin-derived cells were isolated from porcine ear skin after 3 or 4
weeks of primary culture. In vitro osteogenic differentiation of SKPs was observed after co-culturing with a
DMB and fibrin glue scaffold in a non-osteogenic medium. Von Kossa-positive bone minerals were also
noted in the co-cultured medium at 4 weeks. As the culture time progressed, the number of observable
cells increased. Trabecular new bone formation and osteocalcin expression were more pronounced in the
SKP-grafted group compared to the control group.
Conclusion: These findings suggest that autogenous SKP grafting with a DMB and fibrin glue scaffold
can serve as a useful alternative to bone grafting technique.

참고자료

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