개 생균제 사용에 적합한 유산균주의 선발 및 면역활성 평가 (Selection and immunomodulatory evaluation of lactic acid bacteria suitable for use as canine probiotics)

서지정보

· 발행기관 : 대한수의학회
· 수록지 정보 : 대한수의학회지 / 55권 / 2호 / 81 ~ 88페이지
· 저자명 : 박수민, 박호은, 이완규

초록

This study was conducted to isolate lactic acid bacteria (LAB) from dog intestine and identify potential probiotic strains for canine use. One hundred and one LAB were isolated from feces of 20 healthy dogs. Acid, bile,and heat resistance along with adherence to Caco-2 cells and antimicrobial activity against pathogens were examined. To analyze immunomodulative effects, the production of nitric oxide (NO), TNF-α, and IL-1β was measured usingRAW 264.7 macrophages. Additionally, RAW BLUE cells were used to evaluate nuclear factor-κB (NF-κB) generation. Ultimately, three strains were selected as canine probiotics and identified as Lactobacillus reuteri L10, Enterococcusfaecium S33, and Bifidobacterium longum B3 by 16S rRNA sequence analysis. The L10 and S33 strains showed tolerance to pH 2.5 for 2 h, 1.0% Oxgall for 2 h, and 60oC for 5 min. These strains also had strong antimicrobialactivity against Escherichia coli KCTC 1682, Salmonella Enteritidis KCCM 12021, Staphylococcus aureus KCTC 1621, and Listeria monocytogenes KCTC 3569. All three strains exerted better immunomodulatory effects than Lactobacillusrhamnosus GG (LGG), a well-known commercial immunomodulatory strain, based on NO, NF-κB, IL-1β, and TNF-α production. These results suggested that the three selected strains could serve as canine probiotics.

영어초록

This study was conducted to isolate lactic acid bacteria (LAB) from dog intestine and identify potential probiotic strains for canine use. One hundred and one LAB were isolated from feces of 20 healthy dogs. Acid, bile,and heat resistance along with adherence to Caco-2 cells and antimicrobial activity against pathogens were examined. To analyze immunomodulative effects, the production of nitric oxide (NO), TNF-α, and IL-1β was measured usingRAW 264.7 macrophages. Additionally, RAW BLUE cells were used to evaluate nuclear factor-κB (NF-κB) generation. Ultimately, three strains were selected as canine probiotics and identified as Lactobacillus reuteri L10, Enterococcusfaecium S33, and Bifidobacterium longum B3 by 16S rRNA sequence analysis. The L10 and S33 strains showed tolerance to pH 2.5 for 2 h, 1.0% Oxgall for 2 h, and 60oC for 5 min. These strains also had strong antimicrobialactivity against Escherichia coli KCTC 1682, Salmonella Enteritidis KCCM 12021, Staphylococcus aureus KCTC 1621, and Listeria monocytogenes KCTC 3569. All three strains exerted better immunomodulatory effects than Lactobacillusrhamnosus GG (LGG), a well-known commercial immunomodulatory strain, based on NO, NF-κB, IL-1β, and TNF-α production. These results suggested that the three selected strains could serve as canine probiotics.

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