당나귀 사골과 껍질의 항산화기능 및 SK-N-SH세포 보호효과 (Anti-oxidative Activity and the Protective Effect of Donkey’s Bone and Skin Extracts on SK-N-SH Cells)

서지정보

· 발행기관 : 한국생명과학회
· 수록지 정보 : 생명과학회지 / 23권 / 8호 / 1019 ~ 1024페이지
· 저자명 : Dong-Wook Kim, 장애라, 채현석, 김남영

초록

본 실험에서는 당나귀 사골과 껍질을 추출하여 항산화 활성 및 산화적 스트레스로부터 유도되는 신경세포 보호효과를 구명하였다. DBSE의 항산화 활성은 처리 농도가 증가할수록 유의적으로 증가함을 나타내었는데(p<0.05), 40 mg/ml 일 때, 95.43%의 DPPH 라디칼 소거능을 나타내었고, 20 mg/ml일 때, 88.73%의 ABTS 라디칼 소거능을 나타내었으며, 10 mg/ml일 때, 132.53 μM TE의 ORAC을 나타내었다(p<0.05). 신경모세포종 SK-N-SH 세포에 DBSE을 처리한 결과 세포 독성은 나타내지 않았으며, 신경세포에 활성산소종인 H2O2를 처리하여 DBSE의 산화적 스트레스 보호효과를 확인 한 결과, 처리농도의존적으로 신경세포 보호효과를 나타내었다. 따라서 본 연구결과 당나귀 사골과 껍질 추출물은 산화를 억제하고 SK-N-SH 신경세포 보호효과를 보여 생리활성 기능소재로서 이용가능성이 있는 것으로 판단되며 추후 동물실험을 통한 안전성 및 생체대사조절기능과 효과증진기술에 대한 연구가 추가되어야 할 것으로 판단된다.

영어초록

The aims of this study were to determine antioxidation effect and neuroblastoma cell protection effect of donkey’s bone and skin extracts (DBSE). DBSE was extracted by a pressure-cooker for 48 h and lyophilized. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was significantly increased with increased doses of DBSE and 40 mg/ml of DBSE showed 95.43% of the DPPH scavenging effect, which was equivalent to 1 mg/ml of vitamin C. The 2,2'-azino-bis (3-ethylbenzothiazoline- 6- sulphonic acid) (ABTS) radical scavenging activity was also increased in a dose-dependent manner, and 20 mg/ml of DBSE showed 88.73% of the ABTS scavenging effect. The oxygen radical absorbance capacity (μM Trolox equivalent) of DBSE was significantly increased at a concentration of 10 mg/ml, which showed 132.53 μM TE. The viability of oxidatively stressed brain cells induced by 500 μM H2O2 was protected by DBSE at concentrations greater than 50 μM. Cell viability after DBSE treatment at 50 and 100 μg/ml was 53.78 and 54.34 μM TE, respectively. There was no significant difference between both doses; however, 200 and 500 μg/ml of DBSE showed 59.74 and 66.08% of cell viability, respectively indicating that DBSE protected SK-N-SH from oxidation stress. These results suggest that DBSE may have potential to be used as natural antioxidants in food industry, while in vivo evidence is necessary to support DBSE’s in vitro-based antioxidative efficiency.

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