Analysis of epididymal sperm from Korean native bull (Hanwoo) aged at 8 and 15 months before freezing and after thawing

서지정보

· 발행기관 : 한국수정란이식학회
· 수록지 정보 : Journal of Embryo Transfer / 31권 / 2호
· 저자명 : Sung-Sik Kang, Sang-Rae Cho, Ui-Hyung Kim, Chang-Seok Park, Hyeong-Cheol Kim, Ki-Yong Chung, Seok-Dong Lee, Sun-Sik Jang, Gi-jun Jeon, Sidong Kim, Myeong-Suk Lee, Byoung-Chul Yang

목차

Introduction
Materials and Methods
Chemicals
Evaluation of sperm motility by CASA
Preparation of sperm staining and fixing solutions
Staining and fixing of sperm
Evaluation of sperm viability and morphology
Experimental design
Statistical analysis
RESULTS
Experiment 1
Experiment 2
Experiment 3
DISCUSSION
REFERENCES

영어초록

The recovery of epididymal sperm in animals is considered as one of the important tools to preserve high value or endangered species. However, there are no appropriate castrating indicators such as months of age in bull, sperm morphology, and motility, particularly in young Korean native bull (Hanwoo). Therefore, this study aimed to investigate sperm number, morphology, and motility of sperm in the epididymis tail of young Hanwoo bulls at 8 and 15 months of age. After castration, epididymal tails were collected and minced with blades to recover sperm. In experiments 1 and 2, sperm number, morphology, and motility were examined. Total number of sperm and percentage of normal sperm from bulls at 8 months of age was lower than that of bulls at 15 months of age after collection (P<0.05). Percentage of abnormal head, tail, proximal cytoplasmic droplet, dead and damaged acrosome of sperm from bulls at 8 months of age were higher than those of bulls at 15 months of age (P<0.05). In experiment 3, sperm motility from bulls at 8 and 15 months of age were examined before freezing and after thawing. Frozen-thawed sperm at 8 months of age showed low total motility and motile sperm with ≥ 25 μm/sec compared to those at 15 months of age and commercially-used sperm (P<0.05). In conclusion, sperm derived from the epididymal tail of bulls at 8 months of age showed high abnormal morphology and poor motility, which are not adequate for AI and IVF. On the other hand, sperm derived from the epididymal tail of bulls at 15 months of age showed high normal morphology and motility.

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